2. Stem Cell/Wnt TGF-beta/Smad
  3. TGF-beta/Smad
  4. Apitegromab

Apitegromab  (Synonyms: SRK-015)

Cat. No.: HY-P99441 Purity: 99.51%
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Apitegromab (SRK-015) is an anti-promyostatin monoclonal antibody. Apitegromab can be used for the research of neuromuscular disease including spinal muscular atrophy.

For research use only. We do not sell to patients.

CAS No. : 2278276-46-1

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Apitegromab Related Antibodies

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Description

Apitegromab (SRK-015) is an anti-promyostatin monoclonal antibody. Apitegromab can be used for the research of neuromuscular disease including spinal muscular atrophy[1][2].

Isotype

Human IgG4 lambda
Recommend Isotype Controls
Species Reactivity

Human
IC50 & Target

GDF8/Myostatin
In Vitro

Apitegromab (IC50: Human 286 nM, Cynomolgus 626 nM, Rat 178 nM, 16 h) effectively inhibits promyostatin activation, with species-dependent potency differences[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apitegromab Related Antibodies
Parmacokinetics
Species Dose Route Note Clearance (CL) Tmax AUC
Cynomolgus Monkey[2] 10 mg/kg i.v. / 369 μg/mL 1 h 30400 μg·h/mL
Cynomolgus Monkey[2] 100 mg/kg i.v. / 2560 μg/mL 1 h 240000 μg·h/mL
Cynomolgus Monkey[2] 30 mg/kg i.v. / 876 μg/mL 1 h 77200 μg·h/mL
Rat[2] 10 mg/kg i.v. Age: 6-8 weeks 213 μg/mL 1.0 h 16200 μg·h/mL
Rat[2] 100 mg/kg i.v. Age: 6-8 weeks 2360 μg/mL 1 h 170000 μg·h/mL
Rat[2] 30 mg/kg i.v. Age: 6-8 weeks 695 μg/mL 1 h 51100 μg·h/mL
In Vivo

Apitegromab (10-300 mg/kg, i.v., once weekly for 4, 7, or 26 weeks) was well-tolerated in juvenile and adult Sprague Dawley rats as well as adult cynomolgus monkeys, with no severe toxicological effects[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult cynomolgus monkeys (32-48 months)[2]
Dosage: 10, 30, 100 mg/kg
Administration: Intravenous bolus (IV bolus), once weekly for 4 weeks
Result: Did not cause treatment-related adverse effects, increased gastrocnemius and biceps brachii mass (5-25%), but did not exhibit dose dependency, and did not lead to significant histopathological changes or cardiac electrophysiological abnormalities.
Animal Model: Sprague Dawley rats: adult group (6-8 weeks) or juvenile group (postnatal day 21)[2]
Dosage: 10, 30, 100, 300 mg/kg
Administration: Intravenous bolus (IV bolus), once weekly for 4 weeks (adult group), 7 weeks (juvenile group) or 26 weeks (adult group)
Result: Increased body weight independently of food intake, enhanced skeletal muscle mass (biceps brachii and gastrocnemius) by 3-32%, and caused minor clinical chemistry changes (mild increase in total protein and globulin), which were mostly reversible (adult group, administration for 4 weeks).
increased muscle mass by 15-33%, induced mild muscle fiber hypertrophy (histological analysis), and caused minor hematological changes without significant toxicity; it did not produce adverse effects on cardiovascular, neurological, or reproductive systems (adult group, administration for 26 weeks).
increased body weight (significant in females) without impacting growth and development, enhanced skeletal muscle mass (biceps brachii and gastrocnemius) by 13-28%, and did not cause neurobehavioral abnormalities (acoustic startle, locomotor activity, Morris water maze); it had no effects on sperm quality or fertility, indicating normal reproductive function (juvenile group).
Clinical Trial
Gene ID

2660  [NCBI]

Accession
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

145.06 kDa

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Apitegromab]
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • Human IgG4 lambda
Biological Activity
  • Immobilized hu-Latentmyostatin-His can bind Apitegromab. The EC50 for this effect is 25.3 ng/mL.
  • Flow cytometric analysis of 1X106 HeLa cells with Apitegromab (HY-P99441, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa (HY-P99003, blue) was used as the isotype control.
Purity & Documentation

Purity: 99.51%

SDS (251 KB)

COA (232 KB) SDS-PAGE (80 KB) SEC-HPLC (168 KB)

Inhibitory Antibodies User Guide (603 KB)
References
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Apitegromab Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Apitegromab2278276-46-1SRK-015SRK015SRK 015TGF-beta/SmadTransforming growth factor betamonoclonal antibodyanti-promyostatinneuromuscular diseasespinal muscular atrophyInhibitorinhibitorinhibit

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