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Cat. No.: HY-108786

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Biologically Validated

Asfotase alfa

(Synonyms: ENB-0040)

Customer Review

Based on 1 Customer Validation

Asfotase alfa (ENB-0040) is a bone-targeted genetically engineered glycoprotein. Asfotase alfa increases the survival rate, bone mineralization and bone length and prevents mineralization defects of the feet, rib cage, lower limbs, jaw bones in Akp2^/ knockout mice. Asfotase alfa can be used for the research of perinatal, infantile, and juvenile-onset hypophosphatasia (HPP).

For research use only. We do not sell to patients.

Purity: 98.53%
CAS No.: 1174277-80-5
Molecular Weight:161.135 kDa

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Asfotase alfa Chemical Structure

CAS No.: 1174277-80-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Isotype
- Species Reactivity
- In Vivo
- Clinical Trial
- Conjugated
- Reconsititution
- Format
- Verified Bioactivity
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Isotype

Fusion-ALPL-IGHG1 Fc

Species Reactivity

Human

In Vivo

Asfotase alfa (0.5-8.2 mg/kg; s.c. once daily for 43 days) shows dose-response relationships that strongly support the pharmacological efficacy for HPP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Akp2^−/− knockout mice^[1]
Dosage:

0.5, 2.0 and 8.2 mg/kg
Administration:

Subcutaneous injection; once daily for 43 days
Result:

Dose-dependently increased the survival rate of mice, bone mineralization and prevention of mineralization defects of the feet, rib cage, lower limbs, jaw bones, the median survival, body weight, and bone length. Showed a clear relationship between daily dose and achieving a healthy BW and the ED₈₀ (the dose that prevents bone defects in 80% of mice) was 3.2, 2.8 and 2.9 mg/kg/day for these sites. Remained increasing urinary PPi concentrations in all treatment groups.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Format

[ALPL (alkaline phosphatase, TNSALP, tissue non-specific alkaline phosphatase)18-502]2-IGHG1 Fc (Fragment constant)-Asp-Ile-deca-aspartate

Verified Bioactivity

Immobilized Asfotase alfa can bind Anti-Alkaline Phosphatase/ALPL Antibody. The EC₅₀ for this effect is 3.417 ng/mL.

Chemical Information

CAS No. 1174277-80-5
Appearance Liquid
Molecular Weight 161.135 kDa
Color Colorless to light yellow
SMILES

[Asfotase alfa]
Synonyms

ENB-0040
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (256 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

References

[1]. Yadav MC, et al. Dose response of bone-targeted enzyme replacement for murine hypophosphatasia. Bone. 2011 Aug;49(2):250-6. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- Description
- Isotype
- Species Reactivity
- In Vivo
- Clinical Trial
- Conjugated
- Reconsititution
- Format
- Biological Activity
Chemical Information
Purity & Documentation
References

Product Name:
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MCE Japan Authorized Agent:

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Asfotase alfa

HOLO Human Interleukin-2 (IL-2) Detection Kit

Bradford Protein Assay Kit

Cell-ATP Viability Detection Kit

Protein A Agarose

PBS Buffer (1×)

Deep Red Fluorescent Rapid Labeling Kit (AF647）

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Asfotase alfa Related Classifications

HY-108786 Related Classifications