Art. -Nr.: HY-104001

BAY-524

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

BAY-524 is a potent Bub1 kinase inhibitor. BAY-524 inhibits the recombinant catalytic domain of human Bub1 with an IC₅₀ of 450 nM. BAY-524 can be used for the research of anti-cancer in combination with other agents.

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Reinheit: 98.36%
CAS. Nr.: 1445830-39-6
Formel: C₂₄H₂₄F₂N₆O₃
Molecular Weight:482.48

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

BAY-524 Chemical Structure

CAS. Nr.: 1445830-39-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 450 nM (human Bub1)^[1]

In Vitro

BAY-524 inhibits the recombinant catalytic domain of human Bub1 with an IC₅₀ of 450 nM^[1].
BAY-524 (7-10 μM or 0-30 μM; 14 h or 1 h) specifically inhibit Bub1 kinase^[1].
BAY-524 (7 μM , 12 h or 7 μM, 12 μM, 3 h) affects Sgo1 and Sgo2 localization and chromatid cohesion in cells^[1].
BAY-524 (7 μM, 10 μM) affects localization and activity of the CPC^[1].
BAY-524 (7 μM, 2 h) exerts additive effect on centromere association of CPC^[1].
BAY-524 (7 μM, 48 h) marginally affects SAC signaling^[1].
BAY-524 (7 μM, 10 μM) sensitizes cells to low doses of paclitaxel^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

hTERT-RPE1 (RPE1) and HeLa cells
Concentration:

7-10 μM
Incubation Time:

14 h
Result:

Drastically reduced T120 phosphorylation.

Immunofluorescence^[1]

Cell Line:

RPE1 and HeLa cells
Concentration:

0-30 μM
Incubation Time:

1 h
Result:

Reduced histone H2A-T120 phosphorylation.

Chemical Information

CAS. Nr. 1445830-39-6
Appearance Solid
Molecular Weight 482.48
Formel C₂₄H₂₄F₂N₆O₃
Color Off-white to light yellow
SMILES

COC1=CN=C(C2=NN(CC3=C(F)C=C(OCC)C=C3F)C(OC)=C2C)N=C1NC4=CC=NC=C4
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Journal Impact Factor

Albany Medical College

2026.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 166.67 mg/mL (345.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0726 mL

10.3631 mL

20.7262 mL

5 mM

0.4145 mL

2.0726 mL

4.1452 mL

10 mM

0.2073 mL

1.0363 mL

2.0726 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 4.17 mg/mL (8.64 mM); Clear solution

This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 4.17 mg/mL (8.64 mM); Clear solution

This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Baron AP, et al. Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524. Elife. 2016 Feb 17;5. pii: e12187. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0726 mL	10.3631 mL	20.7262 mL	51.8156 mL
	5 mM	0.4145 mL	2.0726 mL	4.1452 mL	10.3631 mL
	10 mM	0.2073 mL	1.0363 mL	2.0726 mL	5.1816 mL
	15 mM	0.1382 mL	0.6909 mL	1.3817 mL	3.4544 mL
	20 mM	0.1036 mL	0.5182 mL	1.0363 mL	2.5908 mL
	25 mM	0.0829 mL	0.4145 mL	0.8290 mL	2.0726 mL
	30 mM	0.0691 mL	0.3454 mL	0.6909 mL	1.7272 mL
	40 mM	0.0518 mL	0.2591 mL	0.5182 mL	1.2954 mL
	50 mM	0.0415 mL	0.2073 mL	0.4145 mL	1.0363 mL
	60 mM	0.0345 mL	0.1727 mL	0.3454 mL	0.8636 mL
	80 mM	0.0259 mL	0.1295 mL	0.2591 mL	0.6477 mL
	100 mM	0.0207 mL	0.1036 mL	0.2073 mL	0.5182 mL

Keywords

BAY-524

1 Cited Publications

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BAY-524

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Heterocyclic Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BAY-524

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BAY-524

Metabolism/Protease Compound Library

Anti-Cancer Compound Library

Heterocyclic Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) BAY-524

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

BAY-524 Related Classifications

HY-104001 Related Classifications