Art. -Nr.: HY-100396

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SQ22536

42 Cited Publications

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Based on 42 publication(s) in Google Scholar

SQ22536 is an effective adenylate cyclase (AC) inhibitor.

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Reinheit: 99.81%
CAS. Nr.: 17318-31-9
Formel: C₉H₁₁N₅O
Molecular Weight:205.22

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

SQ22536 Chemical Structure

CAS. Nr.: 17318-31-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (42)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

SQ22536 is an effective adenylate cyclase (AC) inhibitor.

IC₅₀ & Target

adenylate cyclase (AC)^[1]

In Vitro

SQ22536 (SQ22,536) effectively inhibits the effect of forskolin with respective IC₅₀ values of 5 μM.
Preincubation with graded concentrations of SQ22536 reveals that both SQ22536 effectively inhibits PACAP-induced reporter gene activation with approximate IC₅₀ value of 5 μM.
SQ22536 more potently inhibits forskolin-induced Elk activation (IC₅₀=10 μM) than 8-Br-cAMP-induced Elk activation (IC₅₀=170 μM).
Most notably, there are substantial differences in the reported potencies of SQ22536 to inhibit the activities of recombinant AC5 and AC6, with respective IC₅₀ values of 2 μM and 360 μM. At a greater concentration (500 μM), SQ22536 significantly inhibits neurite elongation due to either forskolin or 8-Br-cAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 17318-31-9
Appearance Solid
Molecular Weight 205.22
Formel C₉H₁₁N₅O
Color White to off-white
SMILES

NC1=C2N=CN(C3OCCC3)C2=NC=N1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (42)

All WBIF

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Journal Impact Factor

Mol Plant

Arabidopsis HSFA1b functions as a heat sensor inhibiting OST1-mediated stomatal closure through its adenylate-cyclase activity. [Abstract]

2025 Sep 1;18(9):1549-1566. PMID: 40745919
Nat Commun

Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions. [Abstract]

2024 Sep 12;15(1):7996. PMID: 39266508
J Exp Clin Cancer Res

GPR65 sensing tumor-derived lactate induces HMGB1 release from TAM via the cAMP/PKA/CREB pathway to promote glioma progression. [Abstract]

2024 Apr 4;43(1):105. PMID: 38576043
Cell Death Dis

P2Y14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. [Abstract]

2020 May 26;11(5):394. PMID: 32457291

SQ22536 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 May 26;11(5):394. [Abstract]

Western blotting shows that the activation of synovial NLRP3 inflammasome signaling is markedly provoked by decreased cAMP in SQ22536-treated P2Y14R-KO rats.

SQ22536 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 May 26;11(5):394. [Abstract]

SQ22536 treatment enhances the colocalization intensity of synovial NLRP3 and ASC in MSU-stimulated P2Y14R-KO rats in immunofluorescence staining.
Genome Biol

Artemisinin alleviates Parkinson's disease by targeting Adcy5-Gch1 axis to trigger a cascade generation of BH4 and dopamine in rats. [Abstract]

2025 Sep 4;26(1):267. PMID: 40908481
Dev Cell

Collective oscillatory signaling in Dictyosteliumdiscoideum acts as a developmental timer initiated by weak coupling of a noisy pulsatile signal. [Abstract]

2024 Dec 5:S1534-5807(24)00698-1. PMID: 39672161
Int J Biol Macromol

An acidic polysaccharide promoting GLP-1 secretion from Dendrobium huoshanense protocorm-like bodies: Structure validation and activity exploration. [Abstract]

2024 Aug 15:134783. PMID: 39153673
Phytomedicine

Tong-Qiao-Huo-Xue Decoction promotes synaptic remodeling via cAMP/PKA/CREB pathway in vascular dementia rats. [Abstract]

2024 Dec:135:156166. PMID: 39461198
Phytomedicine

Red ginseng extracts ameliorate high-fat diet-induced obesity and insulin resistance by activating the intestinal TGR5-mediated bile acids signaling pathway. [Abstract]

2023 Oct:119:154982. PMID: 37531904
J Agric Food Chem

Preventive Effect of Caffeic Acid Phenethyl Ester, an Active Component of Propolis, against TNF-α-Induced Endothelial Dysfunction through the β2-Adrenoceptor-Mediated eNOS/NO Signal Pathway. [Abstract]

2026 Apr 22;74(15):12132-12144. PMID: 41957988
J Agric Food Chem

Galacto-Oligosaccharides Alleviate LPS-Induced Immune Imbalance in Small Intestine through Regulating Gut Microbe Composition and Bile Acid Pool. [Abstract]

2023 Nov 22;71(46):17615-17626. PMID: 37947505
J Agric Food Chem

Polygonatum cyrtonema Hua Polysaccharide Promotes GLP-1 Secretion from Enteroendocrine L-Cells through Sweet Taste Receptor-Mediated cAMP Signaling. [Abstract]

2020 Jun 24;68(25):6864-6872. PMID: 32456438
Prog Neurobiol

Sodium leak channel contributes to neuronal sensitization in neuropathic pain. [Abstract]

2021 Jul:202:102041. PMID: 33766679
J Bone Miner Res

Bone deterioration in response to chronic high-altitude hypoxia is attenuated by a pulsed electromagnetic field via the primary cilium/HIF-1α axis. [Abstract]

2023 Apr;38(4):597-614. PMID: 36680558
Chin Med

Isoforskolin modulates AQP4-SPP1-PIK3C3 related pathway for chronic obstructive pulmonary disease via cAMP signaling. [Abstract]

2023 Oct 10;18(1):128. PMID: 37817209
Int J Mol Sci

Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway Activity. [Abstract]

2023 Jan 16;24(2):1771. PMID: 36675287
Int J Mol Sci

Asprosin in the Paraventricular Nucleus Induces Sympathetic Activation and Pressor Responses via cAMP-Dependent ROS Production. [Abstract]

2022 Oct 20;23(20):12595. PMID: 36293450
Int J Mol Sci

Sinomenine Hydrochloride Promotes TSHR-Dependent Redifferentiation in Papillary Thyroid Cancer. [Abstract]

2022 Sep 14;23(18):10709. PMID: 36142613
Eur J Pharmacol

Tirzepatide reduces intracellular lipid content by promoting the browning of white fat via the cAMP signaling pathway. [Abstract]

2026 Feb 10:1014:178523. PMID: 41506434
Invest Ophthalmol Vis Sci

Effect of Dipyridamole on Experimental Autoimmune Uveitis: Reprogrammed Immune Cell Landscape and Reduced Th17 Pathogenicity. [Abstract]

2025 Dec 1;66(15):16. PMID: 41334959
Int Immunopharmacol

HQL6 serves as a novel P2Y14 receptor antagonist to ameliorate acute gouty arthritis through inhibiting macrophage pyroptosis. [Abstract]

2023 Jan:114:109507. PMID: 36462336
iScience

Celsr3 is required for Purkinje cell maturation and regulates cerebellar postsynaptic plasticity. [Abstract]

2021 Jul 1;24(7):102812. PMID: 34308297
Antiviral Res

Artesunate inhibits PDE4 leading to intracellular cAMP accumulation, reduced ERK/MAPK signaling, and blockade of influenza A virus vRNP nuclear export. [Abstract]

2023 Jul:215:105635. PMID: 37192683
Sci Rep

ADCY4 inhibits cAMP-induced growth of breast cancer by inactivating FAK/AKT and ERK signaling but is frequently silenced by DNA methylation. [Abstract]

2025 Jul 1;15(1):20426. PMID: 40594405
Clin Immunol

Short-chain fatty acid deficiency drives aberrant B cell differentiation in systemic lupus erythematosus. [Abstract]

2026 Jun:285:110706. PMID: 41974394
Exp Cell Res

VPAC2 receptor mediates VIP-potentiated insulin secretion via ion channels in rat pancreatic β cells. [Abstract]

2023 Feb 15;423(2):113471. PMID: 36642263
Dis Model Mech

Glycosphingolipids are linked to elevated neurotransmission and neurodegeneration in a Drosophila model of Niemann Pick type C. [Abstract]

2023 Oct 1;16(10):dmm050206. PMID: 37815467
Front Oncol

Nevirapine Increases Sodium/Iodide Symporter-Mediated Radioiodide Uptake by Activation of TSHR/cAMP/CREB/PAX8 Signaling Pathway in Dedifferentiated Thyroid Cancer. [Abstract]

2020 Mar 31;10:404. PMID: 32300552

SQ22536 purchased from MedChemExpress. Usage Cited in: Front Oncol. 2020 Mar 31;10:404. [Abstract]

The expressions of cAMP, pCREB, PAX8, and NIS in WRO 82-1 and dFTC-133 cells treated with or without 200 μM Nevirapine and SQ22536 (10 μM) for 72 h are determined by western blot and analyzed quantitatively.
Korean J Pain

Perampanel ameliorates nitroglycerin-induced migraine through inhibition of the cAMP/PKA/CREB signaling pathway in the trigeminal ganglion in rats. [Abstract]

2023 Jul 1;36(3):335-346. PMID: 37394274
Arch Biochem Biophys

The odorant (R)-(-)-carvone promotes glucose-stimulated insulin secretion via the olfactory receptor Olfr1259 in pancreatic β-TC6 cells. [Abstract]

2025 Mar 27:768:110404. PMID: 40157529
Neuroscience

Acute Administration of Lactate Exerts Antidepressant-like Effect Through cAMP-dependent Protein Synthesis. [Abstract]

2024 Mar 26:542:11-20. PMID: 38336096
J Cell Biochem

Fsk and IBMX inhibit proliferation and proapoptotic of glioma stem cells via activation of cAMP signaling pathway. [Abstract]

2019 Jan;120(1):321-331. PMID: 30171713

SQ22536 purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Jan;120(1):321-331. [Abstract]

The combination of Fsk and IBMX (Fsk-IBMX) inhibits the expression of cAMP related protein. The results of Western blot in glioma stem cells (GSCs).
J Exp Biol

Olfactory detection of ursodeoxycholic acid (UDCA) in large yellow croaker (Larimichthys crocea): responses, pathways and receptor functions. [Abstract]

2025 Sep 25:jeb.250571. PMID: 40995806
Theriogenology

VEZF1 Suppresses proliferation and induces premature luteinization of porcine granulosa cells. [Abstract]

2026 May 25:263:118009. PMID: 42202716
J Nat Med

Magnolol upregulates CHRM1 to attenuate Amyloid-β-triggered neuronal injury through regulating the cAMP/PKA/CREB pathway. [Abstract]

2022 Jan;76(1):188-199. PMID: 34705126
J Neurophysiol

cAMP-dependent protein kinase signaling is required for ( 2R,6R)-hydroxynorketamine to potentiate hippocampal glutamatergic transmission. [Abstract]

2024 Jan 1;131(1):64-74. PMID: 38050689
bioRxiv

2025 Nov 28.
bioRxiv

2025 April 26.
Res Sq

2025 Apr 08.
bioRxiv

Rapid Hippocampal Synaptic Potentiation Induced by Ketamine Metabolite ( 2R , 6R )-Hydroxynorketamine Persistently Primes Synaptic Plasticity. [Abstract]

2024 Oct 22:2024.10.18.619152. PMID: 39484512
Res Sq

2024 Jun 11.
J Basic Clin Physiol Pharmacol

Intracellular signaling molecules of nerve tissue progenitors as pharmacological targets for treatment of ethanol-induced neurodegeneration. [Abstract]

2021 Feb 8;33(3):305-315. PMID: 33559456

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (487.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 55 mg/mL (268.01 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8728 mL

24.3641 mL

48.7282 mL

5 mM

0.9746 mL

4.8728 mL

9.7456 mL

10 mM

0.4873 mL

2.4364 mL

4.8728 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 25 mg/mL (121.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protokoll

Cell Assay^[1]

HEK293 CRE-luc2P GloResponse luciferase reporter cells are transduced with retroviral vectors expressing rat PAC1hop receptors. Individual cell lines are obtained by limiting dilution cloning, and a clonal PAC₁-expressing line is propagated and used for CRE luciferase assays. In brief, HEK293 CRE-luc2P cells are plated in 96-well plates (10,000 cells in 80 μL media per well) in assay media (DMEM supplemented with 1% fetal bovine serum). One day after plating, cells are treated with AC inhibitors (10 μL in assay media/well) for 30 minutes, followed by agonists (10 μL in assay media/well), and are incubated for 4 hours. Luciferase activity is determined after the addition of 100 μL/well Bright-Glo Luciferase Assay Reagent. Luminescence (RLU) is measured in a Victor3 microtiter plate reader after 2 minutes of agitation at room temperature. Cyclic AMP is measured in NS-1 cells. In brief, NS-1 cells are seeded and grown overnight in 96-well plates. The next day, cells are pretreated for 20 minutes in media containing the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.5 mM) with or without SQ22536. After pretreatment with inhibitors, cells are stimulated with agonists, added as 10× solutions, for an additional 20 minutes. Intracellular cAMP is then assayed using the cAMP Biotrak enzyme immunoassay kit for measurement of nonacetylated cAMP^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Emery AC, et al. A new site and mechanism of action for the widely used adenylate cyclase inhibitor SQ22,536. Mol Pharmacol. 2013 Jan;83(1):95-105. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	4.8728 mL	24.3641 mL	48.7282 mL	121.8205 mL
	5 mM	0.9746 mL	4.8728 mL	9.7456 mL	24.3641 mL
	10 mM	0.4873 mL	2.4364 mL	4.8728 mL	12.1820 mL
	15 mM	0.3249 mL	1.6243 mL	3.2485 mL	8.1214 mL
	20 mM	0.2436 mL	1.2182 mL	2.4364 mL	6.0910 mL
	25 mM	0.1949 mL	0.9746 mL	1.9491 mL	4.8728 mL
	30 mM	0.1624 mL	0.8121 mL	1.6243 mL	4.0607 mL
	40 mM	0.1218 mL	0.6091 mL	1.2182 mL	3.0455 mL
	50 mM	0.0975 mL	0.4873 mL	0.9746 mL	2.4364 mL
	60 mM	0.0812 mL	0.4061 mL	0.8121 mL	2.0303 mL
	80 mM	0.0609 mL	0.3046 mL	0.6091 mL	1.5228 mL
	100 mM	0.0487 mL	0.2436 mL	0.4873 mL	1.2182 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

SQ22536

42 Cited Publications

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SQ22536

GPCR/G Protein Compound Library

Targeted Diversity Library

Human Metabolite Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Lactylation Compound Library

3D Diverse Fragment Library

Publications Citing Use of MedChemExpress (MCE) SQ22536

Biologische Aktivität

Chemical Information

Publications (42)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SQ22536

GPCR/G Protein Compound Library

Targeted Diversity Library

Human Metabolite Library

Heterocyclic Compound Library

Membrane Protein-targeted Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Lactylation Compound Library

3D Diverse Fragment Library

Publications Citing Use of MedChemExpress (MCE) SQ22536

Biologische Aktivität

Chemical Information

Publications (42)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SQ22536 Related Classifications

HY-100396 Related Classifications