Anticancer agent 93
Based on 1 Customer Validation
Anticancer agent 93 is a 4-Hydroxycoumarin derivative. Anticancer agent 93 can inhibit invasion and migration of lung cancer cells by modulating expression of epithelial-mesenchymal transition (EMT) effectors.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.01%
- CAS. Nr.: 94205-22-8
- Formel: C16H10ClNO4
- Molecular Weight:315.71
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
Anticancer agent 93 (compound 4h) (5-15 μM; 24 h) significantly inhibits A549 cell invasion by ~ 50%[1].
Anticancer agent 93 (15 μM; 24-72 h) significantly inhibits A549 cell migration[1].
Anticancer agent 93 (5-15 μM; 4 h) inhibits lung cancer cell motility by downregulating EMT markers[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A549 cells
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Concentration:5, 10, 15 μM
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Incubation Time:24 hours
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Result:Decreased expression of N-cadherin and E-cadherin.
Chemical Information
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CAS. Nr. 94205-22-8
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Appearance Solid
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Molecular Weight 315.71
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Formel C16H10ClNO4
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Color White to off-white
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SMILES
O=C(NC(C1=O)=C(O)C2=C(O1)C=CC=C2)C3=CC=CC(Cl)=C3
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (316.75 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
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Cell invasion
Cell invasion is the ability of cells to migrate from one area to another via the extracellular matrix. Cell invasion is the response of normal and cancer cells to chemical and mechanical stimuli. Before migrating to a new region, the extracellular matrix is degraded by proteases within the cell. Cell invasion often occurs during wound repair, vascularization and inflammation, abnormal tissue invasion, and tumor cell metastasis.
Reinheit & Dokumentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.1675 mL | 15.8373 mL | 31.6746 mL | 79.1866 mL |
| 5 mM | 0.6335 mL | 3.1675 mL | 6.3349 mL | 15.8373 mL | |
| 10 mM | 0.3167 mL | 1.5837 mL | 3.1675 mL | 7.9187 mL | |
| 15 mM | 0.2112 mL | 1.0558 mL | 2.1116 mL | 5.2791 mL | |
| 20 mM | 0.1584 mL | 0.7919 mL | 1.5837 mL | 3.9593 mL | |
| 25 mM | 0.1267 mL | 0.6335 mL | 1.2670 mL | 3.1675 mL | |
| 30 mM | 0.1056 mL | 0.5279 mL | 1.0558 mL | 2.6396 mL | |
| 40 mM | 0.0792 mL | 0.3959 mL | 0.7919 mL | 1.9797 mL | |
| 50 mM | 0.0633 mL | 0.3167 mL | 0.6335 mL | 1.5837 mL | |
| 60 mM | 0.0528 mL | 0.2640 mL | 0.5279 mL | 1.3198 mL | |
| 80 mM | 0.0396 mL | 0.1980 mL | 0.3959 mL | 0.9898 mL | |
| 100 mM | 0.0317 mL | 0.1584 mL | 0.3167 mL | 0.7919 mL |
Anticancer agent 93 Related Classifications
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