Art. -Nr.: HY-13563

Batabulin

(Synonyms: T138067)

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Batabulin (T138067) is an antitumor agent, which binds covalently and selectively to a subset of the β-tubulin isotypes, thereby disrupting microtubule polymerization. Batabulin affects cell morphology and leads to cell-cycle arrest ultimately induces apoptotic cell death.

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Reinheit: 99.89%
CAS. Nr.: 195533-53-0
Formel: C₁₃H₇F₆NO₃S
Molecular Weight:371.26

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Batabulin Chemical Structure

CAS. Nr.: 195533-53-0

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

β-tubulin^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
BEAS-2B	IC₅₀	>40 μM Compound: T138067	Cytotoxicity against human BEAS2B cells assessed as reduction in cell viability after 70 hrs by alamar blue assay Cytotoxicity against human BEAS2B cells assessed as reduction in cell viability after 70 hrs by alamar blue assay	[PMID: 29471119]
BEAS-2B	IC₅₀	12.2 μM Compound: T138067	Antiproliferative activity against human BEAS2B cells after 70 hrs by alamar blue assay Antiproliferative activity against human BEAS2B cells after 70 hrs by alamar blue assay	[PMID: 29471119]
DLD-1	EC₅₀	0.15 μM Compound: 11	Tested for caspase activation activity in colorectal DLD-1 cell line Tested for caspase activation activity in colorectal DLD-1 cell line	[PMID: 12773051]
HBL-100	IC₅₀	>40 μM Compound: T138067	Cytotoxicity against human HBL100 cells assessed as reduction in cell viability after 70 hrs by alamar blue assay Cytotoxicity against human HBL100 cells assessed as reduction in cell viability after 70 hrs by alamar blue assay	[PMID: 29471119]
HBL-100	IC₅₀	0.2 μM Compound: T138067	Antiproliferative activity against human HBL100 cells after 70 hrs by alamar blue assay Antiproliferative activity against human HBL100 cells after 70 hrs by alamar blue assay	[PMID: 29471119]
HeLa	IC₅₀	101 nM Compound: T-138067	Inhibition of growth of human cervical carcinoma (HeLa) cell line. Inhibition of growth of human cervical carcinoma (HeLa) cell line.	[PMID: 11606124]
HUVEC	IC₅₀	16.4 μM Compound: T138067	Cytotoxicity against HUVEC assessed as reduction in cell viability after 70 hrs by alamar blue assay Cytotoxicity against HUVEC assessed as reduction in cell viability after 70 hrs by alamar blue assay	[PMID: 29471119]
HUVEC	IC₅₀	6.3 μM Compound: T138067	Antiproliferative activity against HUVEC after 70 hrs by alamar blue assay Antiproliferative activity against HUVEC after 70 hrs by alamar blue assay	[PMID: 29471119]
MDR	IC₅₀	87 nM Compound: T-138067	Inhibition of growth of human mammary carcinoma (MCF-7) cell line that exhibits the MDR phenotype. Inhibition of growth of human mammary carcinoma (MCF-7) cell line that exhibits the MDR phenotype.	[PMID: 11606124]
MIA PaCa-2	IC₅₀	47.3 μM Compound: T138067	Cytotoxicity against human MIAPaCa2 cells assessed as reduction in cell viability after 70 hrs by alamar blue assay Cytotoxicity against human MIAPaCa2 cells assessed as reduction in cell viability after 70 hrs by alamar blue assay	[PMID: 29471119]
T47D	EC₅₀	0.17 μM Compound: 11	Tested for caspase activation activity in human breast cancer T47D cells Tested for caspase activation activity in human breast cancer T47D cells	[PMID: 12773051]
ZR-75-1	EC₅₀	0.13 μM Compound: 11	Tested for caspase activation activity in human breast cancer ZR-75-1 cells Tested for caspase activation activity in human breast cancer ZR-75-1 cells	[PMID: 12773051]

In Vitro

Batabulin (T138067; 30-300 nM; 24 hours; MCF7 cells) treatment shows approximately 25-30% tetraploid (4n) DNA content in cells, indicating an arrest at the G2/M cell-cycle boundary^[1].
Batabulin (T138067; 30-300 nM; 24-48 hours; MCF7 cells) treatment shows 25-30% apoptosis. After a 48-hr exposure to 100 nM Batabulin, approximately 50-80% of the cell population is undergoing apoptosis^[1].
Batabulin (T138067) binds covalently and selectively to a subset of the β-tubulin isotypes, thereby disrupting microtubule polymerization. Covalent modification occurs at a conserved Cys-239 shared by the β1, β2, and β4 tubulin isotypes. Cells exposed to Batabulin become altered in shape, indicating a collapse of the cytoskeleton, and show an increase in chromosomal ploidy^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:

MCF7 cells
Concentration:

30 nM, 100 nM and 300 nM
Incubation Time:

24 hours
Result:

Showed an arrest at the G2/M cell-cycle boundary.

Apoptosis Analysis^[1]

Cell Line:

MCF7 cells
Concentration:

30 nM, 100 nM and 300 nM
Incubation Time:

24 hours or 48 hours
Result:

25-30% of cells showed the reduced DNA content characteristic of apoptotic cells.

In Vivo

Batabulin (T138067; 40 mg/kg; intraperitoneal injection; once per week; on days 5, 12, and 19; male athymic nude mice) treatment impairs the growth of the drug-sensitive CCRF-CEM tumors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male athymic nude mice (nu/nu) (6-8 week-old, 20-25 g) injected with CCRF-CEM cells^[1]
Dosage:

40 mg/kg
Administration:

Intraperitoneal injection; once per week; on days 5, 12, and 19
Result:

Impaired the growth of the drug-sensitive CCRF-CEM tumors.

Chemical Information

CAS. Nr. 195533-53-0
Appearance Solid
Molecular Weight 371.26
Formel C₁₃H₇F₆NO₃S
Color White to off-white
SMILES

O=S(C1=C(F)C(F)=C(F)C(F)=C1F)(NC2=CC=C(OC)C(F)=C2)=O
Synonyms

T138067
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

Ethanol : 100 mg/mL (269.35 mM; Need ultrasonic)

DMSO : 100 mg/mL (269.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6935 mL

13.4677 mL

26.9353 mL

5 mM

0.5387 mL

2.6935 mL

5.3871 mL

10 mM

0.2694 mL

1.3468 mL

2.6935 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Shan B, et al. Selective, covalent modification of beta-tubulin residue Cys-239 by T138067, an antitumor agent with in vivo efficacy against multidrug-resistant tumors. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5686-91. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / DMSO	1 mM	2.6935 mL	13.4677 mL	26.9353 mL	67.3383 mL
	5 mM	0.5387 mL	2.6935 mL	5.3871 mL	13.4677 mL
	10 mM	0.2694 mL	1.3468 mL	2.6935 mL	6.7338 mL
	15 mM	0.1796 mL	0.8978 mL	1.7957 mL	4.4892 mL
	20 mM	0.1347 mL	0.6734 mL	1.3468 mL	3.3669 mL
	25 mM	0.1077 mL	0.5387 mL	1.0774 mL	2.6935 mL
	30 mM	0.0898 mL	0.4489 mL	0.8978 mL	2.2446 mL
	40 mM	0.0673 mL	0.3367 mL	0.6734 mL	1.6835 mL
	50 mM	0.0539 mL	0.2694 mL	0.5387 mL	1.3468 mL
	60 mM	0.0449 mL	0.2245 mL	0.4489 mL	1.1223 mL
	80 mM	0.0337 mL	0.1683 mL	0.3367 mL	0.8417 mL
	100 mM	0.0269 mL	0.1347 mL	0.2694 mL	0.6734 mL

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Batabulin

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Cytoskeleton Compound Library

Anti-Lung Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Batabulin

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Cytoskeleton Compound Library

Anti-Lung Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Batabulin Related Classifications

HY-13563 Related Classifications