Art. -Nr.: HY-W019543

Disuccinimidyl suberate Crosslinker

16 Cited Publications

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Based on 16 publication(s) in Google Scholar

Disuccinimidyl suberate Crosslinker is an amine-reactive bifunctional protein crosslinker. Disuccinimidyl suberate Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

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Reinheit: 98.0%
CAS. Nr.: 68528-80-3
Formel: C₁₆H₂₀N₂O₈
Molecular Weight:368.34

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Disuccinimidyl suberate Crosslinker Chemical Structure

CAS. Nr.: 68528-80-3

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Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (16)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Disuccinimidyl suberate Crosslinker is an amine-reactive bifunctional protein crosslinker. Disuccinimidyl suberate Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

In Vitro

NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. Disuccinimidyl suberate Crosslinker is first dissolved in an organic solvent such as DMSO, then added to the aqueous crosslinking reaction.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 68528-80-3
Appearance Solid
Molecular Weight 368.34
Formel C₁₆H₂₀N₂O₈
Color White to light yellow
SMILES

O=C(ON1C(CCC1=O)=O)CCCCCCC(ON2C(CCC2=O)=O)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (16)

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Journal Impact Factor

Cell

Red blood cells undergo lytic programmed cell death involving NLRP3. [Abstract]

2025 May 29;188(11):3013-3029.e19. PMID: 40252640
Nat Commun

TMEM25 inhibits monomeric EGFR-mediated STAT3 activation in basal state to suppress triple-negative breast cancer progression. [Abstract]

2023 Apr 24;14(1):2342. PMID: 37095176
Redox Biol

Role of Selenoprotein W in participating in the progression of non-alcoholic fatty liver disease. [Abstract]

2024 May:71:103114. PMID: 38460355
Cancer Lett

HNRNPC lactylation promotes pancreatic cancer progression through mediating the alternative splicing of PAK6. [Abstract]

2025 Dec 22:639:218230. PMID: 41435694
Cell Commun Signal

Homotherapy for heteropathy: therapeutic effect of Butein in NLRP3-driven diseases. [Abstract]

2024 Jun 7;22(1):315. PMID: 38849890
Mater Today Bio

Nanotube topography inhibits NLRP3 inflammasome activation by reducing microtubule glutamylation. [Abstract]

2025 May 10:32:101838. PMID: 40487178
Acta Pharmacol Sin

YOD1 mediates isoproterenol-induced cardiac remodeling by deubiquitinating PKM2 and reducing PKM2 tetramerization in cardiomyocytes. [Abstract]

2025 Jun 11. PMID: 40500343
Dev Cell

A pathological role of O-GlcNAcylation-driven TR11B production and function in lung adenocarcinoma. [Abstract]

2025 Sep 3:S1534-5807(25)00530-1. PMID: 40930100
Mol Med

Extinguishing the flames of inflammation: retardant effect of chlorquinaldol on NLRP3-driven diseases. [Abstract]

2024 Dec 19;30(1):245. PMID: 39701924
Free Radic Biol Med

CAND1 drives intestinal ischemia/reperfusion injury via SCFFBXO6-PKM2-mediated mitochondrial fission and apoptosis. [Abstract]

2026 Jun 15:254:181-195. PMID: 42297194
J Mol Cell Biol

β-alanine alleviates gout by inhibiting NLRP3 inflammasome activation. [Abstract]

2026 Apr 1:mjag004. PMID: 41921068
PLoS Pathog

African swine fever virus pEP364R acts as an important inflammatory-inducing factor to activate NLRP3 inflammasome-mediated pyroptosis by regulating DDX3X. [Abstract]

2026 Feb 25;22(2):e1013874. PMID: 41739885
Cell Signal

Extracellular histone H3 induces macrophage inflammation in acute liver failure via HDAC2 activation and PKM2 subcellular relocalization. [Abstract]

2025 Nov:135:112003. PMID: 40683326
J Inflamm Res

ALDH2 Ameliorates Acute Gouty Arthritis Through Inhibiting NLRP3 Inflammasome and Pyroptosis by Nrf2/ROS Pathway. [Abstract]

2025 Aug 14:18:11095-11108. PMID: 40831518
FASEB J

Hypoxia Exposure Promotes Th17 Cell Differentiation Through Activin A-PKM2 Axis to Exacerbate Autoimmune and Autoinflammatory Diseases. [Abstract]

2025 Jun 30;39(12):e70696. PMID: 40515525
SSRN

2023 Nov 8.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (271.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7149 mL

13.5744 mL

27.1488 mL

5 mM

0.5430 mL

2.7149 mL

5.4298 mL

10 mM

0.2715 mL

1.3574 mL

2.7149 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (246 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7149 mL	13.5744 mL	27.1488 mL	67.8721 mL
	5 mM	0.5430 mL	2.7149 mL	5.4298 mL	13.5744 mL
	10 mM	0.2715 mL	1.3574 mL	2.7149 mL	6.7872 mL
	15 mM	0.1810 mL	0.9050 mL	1.8099 mL	4.5248 mL
	20 mM	0.1357 mL	0.6787 mL	1.3574 mL	3.3936 mL
	25 mM	0.1086 mL	0.5430 mL	1.0860 mL	2.7149 mL
	30 mM	0.0905 mL	0.4525 mL	0.9050 mL	2.2624 mL
	40 mM	0.0679 mL	0.3394 mL	0.6787 mL	1.6968 mL
	50 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3574 mL
	60 mM	0.0452 mL	0.2262 mL	0.4525 mL	1.1312 mL
	80 mM	0.0339 mL	0.1697 mL	0.3394 mL	0.8484 mL
	100 mM	0.0271 mL	0.1357 mL	0.2715 mL	0.6787 mL

Keywords

Disuccinimidyl suberate Crosslinker

16 Cited Publications

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Disuccinimidyl suberate Crosslinker

Publications Citing Use of MedChemExpress (MCE) Disuccinimidyl suberate Crosslinker

Biologische Aktivität

Chemical Information

Publications (16)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Help & FAQs

Complete Stock Solution Preparation Table

Disuccinimidyl suberate Crosslinker Related Classifications

HY-W019543 Related Classifications