Hydroxylunidine
Hydroxylunidine is a quinoline alkaloid that can be isolated from the leaves of Lunasia amara Blanco. Hydroxylunidine has weak antiproliferative activity against HeLa and A2780 cells.
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- CAS. Nr.: 518-59-2
- Formel: C17H21NO6
- Molecular Weight:335.35
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 518-59-2
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Molecular Weight 335.35
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Formel C17H21NO6
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SMILES
CN1C2=C(C=CC3=C2OCO3)C(OC)=C(C1=O)C[C@H](O)C(C)(O)C
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)