Art. -Nr.: HY-111126

K67

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Based on 1 publication(s) in Google Scholar

K67 is a selective the interaction between Keap1 and S349 phosphorylated p62 inhibitor, with an IC₅₀ of 1.5 μM. K67 has a weaker inhibitory effect on the interaction between Keap1 and Nrf2 (IC₅₀ is 6.2 μM). K67 competitively binds to the binding site of Keap1 with p-p62, blocking the abnormal activation of the p62-dependent Nrf2 pathway. K67 inhibits tumor cell proliferation and enhances the sensitivity of hepatocellular carcinoma (HCC) to chemotherapeutic drugs by restoring Keap1-mediated ubiquitination and degradation of Nrf2.

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Reinheit: 98.26%
CAS. Nr.: 2046250-48-8
Formel: C₂₉H₃₀N₂O₇S₂
Molecular Weight:582.69

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

K67 Chemical Structure

CAS. Nr.: 2046250-48-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

K67 (50 μM; 24-72 h) significantly inhibits the proliferation of Huh1 cells and Huh7 cells expressing phospho-mimetic p62 in cell viability assays¹.
K67 (50 μM; 12-48 h) inhibits the expression of Nrf2 target genes in Huh1 cells¹.
K67 (50 μM; 4-36 h respectively) significantly reduces the content of Nrf2 in the nucleus and cytoplasm of Huh1 cells and Keap1 accumulates in a time-dependent manner¹.
K67 (50 μM; treated for 12 h) significantly inhibits the interaction between phosphorylated p62 and Keap1 and promotes the interaction between Nrf2 and Keap1 in immunoprecipitation assays in Huh1 cells¹.
K67 (50 μM; treated for 4 h + 8 h (with the addition of the proteasome inhibitor lactacystin)) significantly promotes the ubiquitination of Nrf2 in Huh7 cells (expressing S351E)¹.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Huh1 cells
Concentration:

50 μM
Incubation Time:

24 h, 48 h, 72 h
Result:

Significantly inhibited the proliferation of Huh1 cells.

RT-PCR^[1]

Cell Line:

Huh1 cells
Concentration:

50 μM
Incubation Time:

12 h, 24 h, 48 h
Result:

Inhibited the expression of Nrf2 target genes in Huh1 cells.

Western Blot Analysis^[1]

Cell Line:

Huh1 cells
Concentration:

50 μM
Incubation Time:

4 h, 12 h, 24 h, 36 h
Result:

Increased the content of Nrf2 in both nuclear and cytosolic fractions of Huh1 cells decreased significantly. Meanwhile, Keap1 accumulated in a time-dependent manner.

Western Blot Analysis^[1]

Cell Line:

Huh1 cells
Concentration:

50 μM
Incubation Time:

12 h
Result:

The interaction between phosphorylated p62 and Keap1 in Huh1 cells was significantly inhibited, while the interaction between Nrf2 and Keap1 was promoted.

Chemical Information

CAS. Nr. 2046250-48-8
Appearance Solid
Molecular Weight 582.69
Formel C₂₉H₃₀N₂O₇S₂
Color Off-white to light yellow
SMILES

CC(CC1=CC(NS(C2=CC=C(C=C2)OCC)(=O)=O)=C3C=CC=CC3=C1NS(C4=CC=C(C=C4)OCC)(=O)=O)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

Adv Sci (Weinh)

Chchd10: A Novel Metabolic Sensor Modulating Adipose Tissue Homeostasis. [Abstract]

2025 Apr;12(15):e2408763. PMID: 39985288

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (171.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7162 mL

8.5809 mL

17.1618 mL

5 mM

0.3432 mL

1.7162 mL

3.4324 mL

10 mM

0.1716 mL

0.8581 mL

1.7162 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 10 mg/mL (17.16 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Saito T, et al. p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming. Nat Commun. 2016 Jun 27;7:12030. [Content Brief]

[2]. Yasuda D, et al. Synthesis of Keap1-phosphorylated p62 and Keap1-Nrf2 protein-protein interaction inhibitors and their inhibitory activity. Bioorg Med Chem Lett. 2016 Dec 15;26(24):5956-5959. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7162 mL	8.5809 mL	17.1618 mL	42.9045 mL
	5 mM	0.3432 mL	1.7162 mL	3.4324 mL	8.5809 mL
	10 mM	0.1716 mL	0.8581 mL	1.7162 mL	4.2904 mL
	15 mM	0.1144 mL	0.5721 mL	1.1441 mL	2.8603 mL
	20 mM	0.0858 mL	0.4290 mL	0.8581 mL	2.1452 mL
	25 mM	0.0686 mL	0.3432 mL	0.6865 mL	1.7162 mL
	30 mM	0.0572 mL	0.2860 mL	0.5721 mL	1.4301 mL
	40 mM	0.0429 mL	0.2145 mL	0.4290 mL	1.0726 mL
	50 mM	0.0343 mL	0.1716 mL	0.3432 mL	0.8581 mL
	60 mM	0.0286 mL	0.1430 mL	0.2860 mL	0.7151 mL
	80 mM	0.0215 mL	0.1073 mL	0.2145 mL	0.5363 mL
	100 mM	0.0172 mL	0.0858 mL	0.1716 mL	0.4290 mL

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K67

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Anti-Cancer Metabolism Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Highly Selective Inhibitors Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Mitophagy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) K67

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

K67

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Anti-Cancer Metabolism Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Highly Selective Inhibitors Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Mitophagy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) K67

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

K67 Related Classifications

HY-111126 Related Classifications