MQA-P TFA

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. MQA-P TFA is dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution (1.0 mM).
2. For imaging of ONOO^- in live cells：
HeLa cells are incubated with MQA-P (5 μM) for 30 min as control; pretreated with SIN-1 (HY-126849; 100 μM) for 30 min and then incubated with MQA-P (5 μM) for another 30 min. The fluorescence images are obtained on a confocal laser scanning microscope with a green channel (λ_ex = 405nm, λ_em = 550-670 nm).
3. For imaging of viscosity in live cells：
HeLa cells were incubated with MQA-P (5 μM) for 30 min as control; pretreated with Monensin (HY-N4302; 10 μM) for 30 min and then incubated with MQA-P (5 μM) for another 30min. The fluorescence images are obtained on a confocal laser scanning microscope with a red channel (λ_ex = 561 nm, λ_em = 680-750 nm).
4. For dual-channel imaging of ONOO^-, viscosity and polarity during ferroptosis：
HeLa cells are incubated with MQA-P (5 μM) for 30 min as control; pretreated with Erastin (HY-15763; 50 μM) for 30 min and then incubated with MQA-P (5 μM) for another 30 min. The fluorescence images are obtained on a confocal laser scanning microscope with a green channel (λ_ex = 405nm, λ_em = 550-670 nm) for ONOO^- and a red channel (λ_ex = 561 nm, λ_em = 680-750 nm) for viscosity and polarity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.