MRC71
MRC71 is a TRIMTAC degrader that recruits endogenous TRIM21 E3 ligase at one end and covalently binds to HaloTag-fusion target proteins at the other end. MRC71 selectively degrades oligomeric CAV1- and Cavin1-mEGFP-Halo, but not monomeric mEGFP-Halo, and exhibits a typical concentration-dependent degradation profile with a hook effect. MRC71 can be used to verify TRIMTAC ternary complex formation and state-selective degradation in cells.
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- CAS. Nr.: 3070832-68-4
- Formel: C43H66ClN5O8
- Molecular Weight:816.47
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Biologische Aktivität
Beschreibung
In Vitro
MRC71 (2.5-20 μM) drives the formation of a ternary complex between purified TRIM21 PRYSPRY and HaloTag protein in vitro, with a characteristic hook effect occurring at excessively high compound concentrations[1].
MRC71 (0.001-100 μM; 2 h) induces the formation of a dose-dependent ternary complex between full-length TRIM21 and H2B-mEGFP-Halo in RPE-1 cells, with a hook effect occurring at excessively high compound concentrations[1].
MRC71 (0.01-100 μM; 48 h) is a TRIM21-dependent TRIMTAC that selectively degrades oligomerized CAV1-mEGFP-Halo and Cavin1-mEGFP-Halo in RPE-1 cells, but does not affect monomeric mEGFP-Halo[1].
MRC71 (2.5 μM; 24 h) selectively degrades membrane-associated oligomeric Cavin1-mEGFP-Halo via the proteasome in RPE-1 cells, while preserving the cytoplasmic monomer pool and smaller Cavin1-free cleavage products[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 3070832-68-4
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Molecular Weight 816.47
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Formel C43H66ClN5O8
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SMILES
O=C(C[C@H](N)C1=C(OC)C=CC=C1)N(C2)[C@H](C(N[C@@H](CC3=CC=NC=C3)C(NCCOCCOCCOCCOCCCCCCCl)=O)=O)C[C@H]2C4CCCCC4
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
MRC71 Related Classifications
HY-180547 Related Classifications
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