Art. -Nr.: HY-173020

W23-1006

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W23-1006 is a selective and covalent ALKBH5 inhibitor. W23-1006 binds to the ALKBH5 C200 residue with an IC₅₀ value of 3.848 μM. W23-1006 shows ~30- and 8-fold stronger inhibitory activity than that against FTO and ALKBH3, respectively. W23-1006 can be used for the study of triple-negative breast cancer (TNBC).

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Reinheit: 97.15%
CAS. Nr.: 3035498-92-8
Formel: C₁₇H₁₂BrN₃O₅
Molecular Weight:418.20

Speicherung:

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

W23-1006 Chemical Structure

CAS. Nr.: 3035498-92-8

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

W23-1006 (5 μM; 24 h) significantly increases the m^A abundance in MDA-MB-231 and BT549 cells^[1].
W23-1006 (3.16-31.6 μM; 24 h) inhibits the cell viability of MDA-MB-231 and BT549^[1].
W23-1006 (5 μM) could obviously reduce TNBC cell colonization area and suppress cell migration^[1].
W23-1006 (5-10 μM) induces the apoptosis and decreases the G2/M phase of MDA-MB-231 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

MDA-MB-231 and BT549 cells
Concentration:

5 μM
Incubation Time:

24 h
Result:

The m^A abundance significantly increased in cells.

Cell Viability Assay^[1]

Cell Line:

MDA-MB-231 and BT549 cells
Concentration:

3.16 μM, 10 μM, 31.62 μM
Incubation Time:

24 h
Result:

Inhibited cell viability of MDA-MB-231 and BT549 with IC₅₀ values of 10.31 μM and 6.338 μM, respectively.

In Vivo

W23-1006 (25 mg/kg; intraperitoneal injection (i.p.); once daily; for 14 days) exerts antitumor efects on breast cancer in vivo^[1].
W23-1006 (25 mg/kg; i.p.; once daily; for 10 days) could obviously inhibit TNBC lung metastasis^[1].
W23-1006 (single dosage at 25 mg/kg; i.p.) shows the t_1/2 ranged from 0.509 to 0.983 h, and the C_max in plasma (in the range of 1715-2108 ng/mL) is reached 15 min in mice^[1].
Neither 100 mg/kg nor 250 mg/kg dosage of W23-1006 (i.p.) causes adverse events or death in KM mice (22-27g)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Immunodefcient female mouse was subcutaneously injected MDA-MB-231 cells^[1].
Dosage:

25 mg/kg (5% DMSO, 10% Kolliphor HS 15, and 85% saline)
Administration:

Intraperitoneal injection; once daily; for 14 days
Result:

Apparently reduced the tumor volume and weight.
Strongly reduced the FN1 and Ki-67 protein expression level.

Animal Model:

Immunodefcient female mouse injected MDA-MB-231^LMF3 through the tail vein^[1].
Dosage:

25 mg/kg
Administration:

Intraperitoneal injection; once daily; for 10 days
Result:

Inhibited TNBC lung metastasis.
No apparent pathologic abnormality was observed in the HEstaining results of multiple organs, including heart, liver,spleen, lung, and kidney.
There was no signifcant diference in glutamic pyruvic transaminase(ALT) and glutamic oxaloacetic transaminase (AST) levels in mouse serum, did not infuence mice liver function.

Chemical Information

CAS. Nr. 3035498-92-8
Appearance Solid
Molecular Weight 418.20
Formel C₁₇H₁₂BrN₃O₅
Color Brown to reddish brown
SMILES

O=C(/C1=C\C2=CC([N+]([O-])=O)=CC(Br)=C2)NN(C3=CC=C(C=C3)OC)C1=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (239.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3912 mL

11.9560 mL

23.9120 mL

5 mM

0.4782 mL

2.3912 mL

4.7824 mL

10 mM

0.2391 mL

1.1956 mL

2.3912 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Yang X, et al. Discovery of a Novel Selective and Cell-Active N6-Methyladenosine RNA Demethylase ALKBH5 Inhibitor. J Med Chem. 2025 Feb 27;68(4):4133-4147. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3912 mL	11.9560 mL	23.9120 mL	59.7800 mL
	5 mM	0.4782 mL	2.3912 mL	4.7824 mL	11.9560 mL
	10 mM	0.2391 mL	1.1956 mL	2.3912 mL	5.9780 mL
	15 mM	0.1594 mL	0.7971 mL	1.5941 mL	3.9853 mL
	20 mM	0.1196 mL	0.5978 mL	1.1956 mL	2.9890 mL
	25 mM	0.0956 mL	0.4782 mL	0.9565 mL	2.3912 mL
	30 mM	0.0797 mL	0.3985 mL	0.7971 mL	1.9927 mL
	40 mM	0.0598 mL	0.2989 mL	0.5978 mL	1.4945 mL
	50 mM	0.0478 mL	0.2391 mL	0.4782 mL	1.1956 mL
	60 mM	0.0399 mL	0.1993 mL	0.3985 mL	0.9963 mL
	80 mM	0.0299 mL	0.1495 mL	0.2989 mL	0.7473 mL
	100 mM	0.0239 mL	0.1196 mL	0.2391 mL	0.5978 mL

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W23-1006

Apoptosis Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Anti-Breast Cancer Compound Library

Membrane Protein-targeted Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

W23-1006

Apoptosis Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Anti-Breast Cancer Compound Library

Membrane Protein-targeted Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

W23-1006 Related Classifications

HY-173020 Related Classifications