DiSC3(5)
Based on 15 publication(s) in Google Scholar
DiSC3(5) is a fluorescent probe commonly used as a tracer dye to evaluate mitochondrial membrane potential. The excitation/emission wavelength of DiSC3(5) is up to 622/670 nm. DiSC3(5) can inhibit the respiratory system associated with mitochondrial NAD, and the IC50 value is 8 μM. DiSC3(5) in the presence of Na+/K+-ATPase inhibitor ouabain 2 can induce membrane hyperpolarization of Ehrlich ascites tumor cells.
For research use only. We do not sell to patients.
- Purity: 99.46%
- CAS No.: 53213-94-8
- Formula: C25H27IN2S2
- Molecular Weight:546.53
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DiSC3(5)
More- Int J Biol Macromol. 2026 Jan:336:149288. [Abstract]
- ACS Appl Mater Interfaces. 2025 Sep 3;17(35):49165-49182. [Abstract]
- ACS Appl Mater Interfaces. 2024 Jun 19;16(24):30833-30846. [Abstract]
- Cell Rep. 2025 Sep 2;44(9):116215. [Abstract]
- Virulence. 2026 Dec 31;17(1):2646808. [Abstract]
- Biomacromolecules. 2024 Aug 12;25(8):5138-5148. [Abstract]
- Gels. 2025 Jan 13;11(1):63. [Abstract]
- Int J Food Microbiol. 2025 Jan 23:431:111084. [Abstract]
- Int J Mol Sci. 2024 Sep 15;25(18):9949. [Abstract]
- Int J Antimicrob Agents. 2024 Sep 16:107338. [Abstract]
- Antibiotics (Basel). 2024 Sep 13;13(9):882. [Abstract]
- ACS Omega. 2026 Feb 9;11(7):12450-12460. [Abstract]
- BMC Microbiol. 2025 Nov 11;25(1):728. [Abstract]
- AMB Express. 2024 Dec 24;14(1):141. [Abstract]
- SSRN. 2025 Dec 22.
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of DiSC3(5) membrane staining solution:
1.1 Preparation of DMSO or EtOH reserve solution: The reserve solution should be prepared in DMSO or EtOH at 1-5 mM.
Note 1) : The unused portion of the reserve solution should be stored at -20 ° C. Avoid repeated freezing/thawing cycles.
1.2 Prepare the working solution: Dilute the reserve solution into a suitable buffer, such as serum-free medium, HBSS or PBS, to make a working solution of 1 to 5 uM.
Note 2) : For different cell types and/or experimental conditions, the concentration of the working solution should be determined according to experience. It is recommended to test at concentrations at least in excess of the ten-fold range.
2. Dye the cells into a suspension:
2.1 The suspended cell density in the dye working solution was 1×106/ mL.
2.2 Incubate at 37°C for 2-20 minutes. The culture time depends on the cell type. It is incubated first for 20 minutes and then optimized as needed to obtain even labeling.
2.3 Centrifuge the labeled suspension tubes at 1000 to 1500rpm for 5 minutes.
2.4 Remove the supernatant and gently re-suspend the cells in a preheated (37°C) growth medium.
2.5 Wash twice according to steps 2.3 and 2.4.
3. Staining adherent cells:
3.1 The adherent cells were cultured on a sterile glass cover slide.
3.2 Remove the cover glass from the growth medium and gently drain the excess medium. Place the cover glass in the humidity box.
3.3 Transfer the 100 μL dye working solution to the corner of the cover glass and stir gently until all cells are covered.
3.4 Incubate the cover glass at 37°C for 2-20 minutes. The culture time varies according to cell type. Incubation is started for 20 minutes and then optimized as needed to obtain even labeling.
3.5 Drain the dye working solution and clean the cover glass with growth medium two to three times. For each wash cycle, cover the cells with preheated growth medium, incubate for 5-10 minutes, and then drain the medium.
4. Microscope inspection.
5. Flow cytometry detection:
Dis-labeled cells can be analyzed using conventional FL3 flow cytometry detection channels.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
670
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Chemical Information
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CAS No. 53213-94-8
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Appearance Solid
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Molecular Weight 546.53
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Formula C25H27IN2S2
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Color Blue to dark blue
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SMILES
CCCN1/C(SC2=CC=CC=C12)=C\C=C\C=C\C3=[N+](CCC)C4=CC=CC=C4S3.[I-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (15)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
Bacteriophage lysin P26ly combats multidrug-resistant Escherichia coli O157 and methicillin-resistant Staphylococcus aureus via cell wall degradation and membrane disruption. [Abstract]2026 Jan:336:149288. PMID: 41317755 -
ACS Appl Mater Interfaces
Synergistic Antibacterial Strategy of AIEgens Photosensitizer CPTQ for Photodynamic Therapy and Ferroptosis-like Bacterial Death to Prevent Dental Caries. [Abstract]2025 Sep 3;17(35):49165-49182. PMID: 40849884 -
ACS Appl Mater Interfaces
Membrane-Anchored Aggregation-Induced Emission Luminogens: Accurate Labeling and Efficient Photodynamic Inactivation of Streptococcus mutans in Anticaries Therapy. [Abstract]2024 Jun 19;16(24):30833-30846. PMID: 38842123 -
Cell Rep
2025 Sep 2;44(9):116215. PMID: 40906555 -
Virulence
Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii. [Abstract]2026 Dec 31;17(1):2646808. PMID: 41838520 -
Biomacromolecules
Film-Forming Capability and Antibacterial Activity of Surface-Deacetylated Chitin Nanocrystals: Role of Degree of Deacetylation. [Abstract]2024 Aug 12;25(8):5138-5148. PMID: 39007299 -
Gels
Self-Assembled Peptide Hydrogels PPI45 and PPI47: Novel Drug Candidates for Staphylococcus aureus Infection Treatment. [Abstract]2025 Jan 13;11(1):63. PMID: 39852034
DiSC3(5) purchased from MedChemExpress. Usage Cited in: Gels. 2025 Jan 13;11(1):63. [Abstract]
Effects of PPI42 (1× MIC) on the membrane potential of S. aureus ATCC 43300. The fluorescent probe DiSC3(5) (0.5 mM) was co-incubated with PBS (108 CFU/mL) of S. aureus for 30 min at 37℃.
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Int J Food Microbiol
Antibacterial activity and multi-target mechanism of harmane against Escherichia coli O157:H7 and its application on ready-to-eat leafy greens. [Abstract]2025 Jan 23:431:111084. PMID: 39862743 -
Int J Mol Sci
Transcriptomic and Biochemical Analysis of the Antimicrobial Mechanism of Lipopeptide Iturin W against Staphylococcus aureus. [Abstract]2024 Sep 15;25(18):9949. PMID: 39337437
DiSC3(5) purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Sep 15;25(18):9949. [Abstract]
Overnight-cultured Staphylococcus aureus cells were diluted with fresh LB medium to an OD600 of 0.3. The diluted cells were then mixed with 10 μM DiSC3(5) and aliquoted into black-bottomed 96-well plates. The mixture was incubated at room temperature until the fluorescence value stabilized. Then, different concentrations of C14 iturobrine W were added, and incubation was continued for 30 minutes. The membrane potential changes induced by different concentrations of C14 iturobrine W were detected.
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Int J Antimicrob Agents
Isobavachalcone enhances sensitivity of colistin-resistant Klebsiella pneumoniae: In vitro and in vivo proof-of-concept studies. [Abstract]2024 Sep 16:107338. PMID: 39293773 -
Antibiotics (Basel)
2024 Sep 13;13(9):882. PMID: 39335055 -
ACS Omega
High-Throughput Screening of FDA-Approved Drugs for Antibacterial and Antibiofilm Activities Against Multidrug-resistant Pseudomonas aeruginosa. [Abstract]2026 Feb 9;11(7):12450-12460. PMID: 41768696 -
BMC Microbiol
YZ462 exhibits potent antibacterial activity against methicillin-resistant Staphylococcus aureus through bacterial membrane disruption. [Abstract]2025 Nov 11;25(1):728. PMID: 41219701
DiSC3(5) purchased from MedChemExpress. Usage Cited in: BMC Microbiol. 2025 Nov 11;25(1):728. [Abstract]
Real-time monitoring of inner membrane potential changes in S. aureus ATCC6538 using DiSC3(5) (0.4 μM, 60 min) probe. The blank control was bacteria without drug treatment, and melittin was used as a positive control.
DiSC3(5) purchased from MedChemExpress. Usage Cited in: BMC Microbiol. 2025 Nov 11;25(1):728. [Abstract]
Real-time monitoring of inner membrane potential changes in S. aureus MRS153 using DiSC3(5) (0.4 μM, 60 min) probe. The blank control was bacteria without drug treatment, and melittin was used as a positive control.
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AMB Express
Repurposing pinaverium bromide against Staphylococcus and its biofilms with new mechanisms. [Abstract]2024 Dec 24;14(1):141. PMID: 39718732
DiSC3(5) purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141. [Abstract]
Fluorescence unit quantification of DiSC3(5) (2 μM, 30 min) probe after treated with PVB for 30 s. Melittin (2 µg/mL) was used as control.
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Solvent & Solubility
DMSO : 5.83 mg/mL (10.67 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Yamamoto T, et al. Multiple effects of DiS-C3(5) on mitochondrial structure and function. Eur J Biochem. 2004;271(17):3573-3579. [Content Brief]
[2]. Sims PJ, et al. Studies on the mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles. Biochemistry. 1974 Jul 30;13(16):3315-30. [Content Brief]
[3]. Smith TC, et al. The effect of the fluorescent probe, 3,3'-dipropylthiodicarbocyanine iodide, on the membrane potential of Ehrlich ascites tumor cells. Biochem Biophys Res Commun. 1980 Jul 31;95(2):722-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8297 mL | 9.1486 mL | 18.2973 mL | 45.7431 mL |
| 5 mM | 0.3659 mL | 1.8297 mL | 3.6595 mL | 9.1486 mL | |
| 10 mM | 0.1830 mL | 0.9149 mL | 1.8297 mL | 4.5743 mL |
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