ATTO 665

Phage clones labeled with ATTO 665 (10¹³ phage particles; 15 min) selectively bind to their respective Candida species, producing strong surface-associated fluorescence as observed by fluorescence microscopy^[4].

ATTO 665 (15 mg dissolved in 200 µL DMSO; 4 h) is conjugated to chitosan to afford 3.2 g of blue ATTO 665-labeled chitosan in 82% yield^[1].
ATTO 665 (0.03 μM; 95°C for 30 s, followed by 40 cycles of 96°C for 1 min and 55°C for 45 s) is added to the second-round real-time PCR premix for background monitoring^[2].
ATTO 665 serves as a single energy acceptor in single-molecule FRET experiments using light-harvesting nanoparticles, achieving a FRET efficiency of 51% and enabling single DNA/RNA detection through target-displacement color switching with an LOD of 2 pM^[3].
ATTO 665 (2.5 mg/mL; 60 min) is used to label M13 phage coat proteins in PBS-NaHCO₃ at pH 8.3 at room temperature, targeting approximately 300 dye molecules per phage^[4].
ATTO 665 (5 × 10^-7 M; 0-20 ns) has a ground-truth lifetime of 2.9 ns and is resolved by Kernelizing-PARAFAC-ALS, yielding a 15-point sampled lifetime of 2.8 ns with a concentration spectral metric of 0.88, whereas PowerSlicing gives 2.64 ns and 0.83^[5].