PIPES
Based on 1 Customer Validation
PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 5625-37-6
- Formula: C8H18N2O6S2
- Molecular Weight:302.37
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
To prepare the pH PIPES buffer, 173 g of 1,4-piperazinediethanesulfonic acid are dissolved into 1 L of deionized water. The pH of the PIPES buffer is adjusted to 6.8 by adding pellets of sodium hydroxide. PIPES can be used to PIPES buffer, it can prevent the glutaraldehyde fixation induced lipid loss and artifacts[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5625-37-6
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Appearance Solid
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Molecular Weight 302.37
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Formula C8H18N2O6S2
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Color White to off-white
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SMILES
O=S(CCN1CCN(CCS(=O)(O)=O)CC1)(O)=O
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Synonyms
1,4-Piperazinediethanesulfonic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
1 M NaOH : 100 mg/mL (330.72 mM; ultrasonic and warming and heat to 60°C)
H2O : 1 mg/mL (3.31 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 2 mg/mL (6.61 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (270 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / 1 M NaOH | 1 mM | 3.3072 mL | 16.5360 mL | 33.0721 mL | 82.6802 mL |
| 1 M NaOH | 5 mM | 0.6614 mL | 3.3072 mL | 6.6144 mL | 16.5360 mL |
| 10 mM | 0.3307 mL | 1.6536 mL | 3.3072 mL | 8.2680 mL | |
| 15 mM | 0.2205 mL | 1.1024 mL | 2.2048 mL | 5.5120 mL | |
| 20 mM | 0.1654 mL | 0.8268 mL | 1.6536 mL | 4.1340 mL | |
| 25 mM | 0.1323 mL | 0.6614 mL | 1.3229 mL | 3.3072 mL | |
| 30 mM | 0.1102 mL | 0.5512 mL | 1.1024 mL | 2.7560 mL | |
| 40 mM | 0.0827 mL | 0.4134 mL | 0.8268 mL | 2.0670 mL | |
| 50 mM | 0.0661 mL | 0.3307 mL | 0.6614 mL | 1.6536 mL | |
| 60 mM | 0.0551 mL | 0.2756 mL | 0.5512 mL | 1.3780 mL | |
| 80 mM | 0.0413 mL | 0.2067 mL | 0.4134 mL | 1.0335 mL | |
| 100 mM | 0.0331 mL | 0.1654 mL | 0.3307 mL | 0.8268 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
PIPES Related Classifications
HY-D0875 Related Classifications
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