Betavulgarin
Based on 1 Customer Validation
Betavulgarin is an anticancer agent. Betavulgarin can be isolated from Sugar Beet (Beta vulgaris). Betavulgarin suppresses the proliferation, migration, colony formation, and mammosphere formation of breast cancer cells, and reduces the size of the CD44+/CD24− subpopulation and the expression of the self-renewal- related genes C-Myc, Nanog and Oct4. Betavulgarin promotes BCSCs death through the regulation of Stat3/Sox2 signaling.
For research use only. We do not sell to patients.
- Purity : 98.49%
- CAS No.: 51068-94-1
- Formula: C17H12O6
- Molecular Weight:312.27
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
p-STAT3 |
In Vitro
Betavulgarin (50-500 μM, 24 h) significantly suppresses the proliferation, migration, colony formation, and mammosphere formation of MDA-MB-231 cells, MCF-7 cells[1].
Betavulgarin (200 μM, 24 h) reduces the size of the CD44+/CD24− subpopulation with the cell fraction from 90.4% to 57.2% in MDA-MB-231 cells[1].
Betavulgarin (200 μM, 48 h) inhibits the translocation of Stat3 and decreases the total protein level and nuclear protein level of p-Stat3 as well as the mRNA and protein levels of SOX2 in MDA-MB-231-derived mammospheres[1].
Betavulgarin (200 μM, 48 h) significantly inhibits the DNA-binding capacity of Stat3 in MDA-MB-231-derived mammosphere[1].
Betavulgarin (24-72 h) reduces the mRNA expression of the self-renewal- related genes, C-Myc, Nanog, and Oct4 in BCSCs-derived mammospheres, and inhibits mammosphere growth[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MDA-MB-231 cells, MCF-7 cells
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Concentration:50, 100, 200, 300, 400, 500 μM
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Incubation Time:24 h
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Result:Suppressed the proliferation of MDA-MB-231 and MCF-7 cells at ≥ 100 µM and 50 µM, respectively.
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Cell Line:MDA-MB-231 cells, MCF-7 cells
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Concentration:100 μM
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Incubation Time:24 h
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Result:Inhibited migration of MDA-MB-231 and MCF-7 cells.
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Cell Line:MDA-MB-231 cells, MCF-7 cells
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Concentration:200 μM
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Incubation Time:48 h
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Result:Inhibited the translocation of Stat3, and significantly decreased the total protein levels and nuclear protein level of Stat3 and p-Stat3, as well as the protein levels of SOX2 in MDA-MB-231-derived mammospheres.
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Cell Line:MDA-MB-231 cells, MCF-7 cells
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Concentration:200 μM
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Incubation Time:48 h
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Result:Reduced the level of nuclear pStat3 in MDA-MB-231 cells.
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Cell Line:MDA-MB-231 cells
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Concentration:200 μM
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Incubation Time:48 h
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Result:Reduced the mRNA level of SOX2 in MDA-MB-231-derived mammospheres.
Chemical Information
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CAS No. 51068-94-1
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Appearance Solid
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Molecular Weight 312.27
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Formula C17H12O6
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Color White to off-white
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SMILES
O=C1C(C2=C(O)C=CC=C2)=COC3=C1C(OC)=C4OCOC4=C3
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (320.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
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Soft Agar Colony Formation Assay
Soft agar colony formation assay measures anchorage-independent growth, in which transformed or tumorigenic cells proliferate as colonies in a semisolid agar matrix while many non-transformed adherent cells fail to proliferate without attachment; classic studies showed that growth in semisolid medium correlates with tumorigenicity in nude mice, and later protocol papers describe the method as a stringent in vitro assay for malignant transformation. The readout is the number, size, morphology, or signal intensity of colonies formed within agar after incubation; published formats include manual colony counting after staining, 96-well or 384-well quantitative formats, DNA-binding dye detection, MTT/tetrazolium-based detection, digital image analysis, and PCR-based marker detection from soft agar cultures.
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Colony Formation (Clonogenic) Assay
The clonogenic (colony formation) assay measures the ability of a single cell to retain reproductive viability and form a macroscopic colony, typically defined as a cluster derived from one progenitor cell after a defined growth period. This assay is widely used to evaluate cell survival after exposure to ionizing radiation or cytotoxic treatments and is considered a standard method in radiation biology for generating dose-response relationships of reproductive cell death. Colony formation reflects long-term proliferative capacity rather than short-term metabolic activity, and survival is quantified by comparing treated versus untreated conditions based on colony number and derived survival fractions.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
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hPSC maintenance and expansion
This protocol maintains and expands human pluripotent stem cells under feeder-free, chemically defined conditions using E8 medium and vitronectin-coated culture surfaces; the readout is sustained adherent colony growth with undifferentiated morphology and retained pluripotency-marker expression during serial passaging. E8-based hPSC culture relies on defined soluble factors and matrix-dependent adhesion rather than feeder cells; vitronectin supports hPSC attachment through integrin-mediated interactions, and EDTA passaging dissociates colonies as small aggregates without enzymatic digestion, centrifugation, or routine ROCK-inhibitor treatment.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.2024 mL | 16.0118 mL | 32.0236 mL | 80.0589 mL |
| 5 mM | 0.6405 mL | 3.2024 mL | 6.4047 mL | 16.0118 mL | |
| 10 mM | 0.3202 mL | 1.6012 mL | 3.2024 mL | 8.0059 mL | |
| 15 mM | 0.2135 mL | 1.0675 mL | 2.1349 mL | 5.3373 mL | |
| 20 mM | 0.1601 mL | 0.8006 mL | 1.6012 mL | 4.0029 mL | |
| 25 mM | 0.1281 mL | 0.6405 mL | 1.2809 mL | 3.2024 mL | |
| 30 mM | 0.1067 mL | 0.5337 mL | 1.0675 mL | 2.6686 mL | |
| 40 mM | 0.0801 mL | 0.4003 mL | 0.8006 mL | 2.0015 mL | |
| 50 mM | 0.0640 mL | 0.3202 mL | 0.6405 mL | 1.6012 mL | |
| 60 mM | 0.0534 mL | 0.2669 mL | 0.5337 mL | 1.3343 mL | |
| 80 mM | 0.0400 mL | 0.2001 mL | 0.4003 mL | 1.0007 mL | |
| 100 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8006 mL |
Betavulgarin Related Classifications
HY-N15201 Related Classifications
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