Biotin Azide Plus 

Biotin Azide Plus is an oxazolidine reagent that integrates azide-biotin click chemistry and a photocleavable linker arm. Biotin Azide Plus not only reacts with biotin thioether to form stable sulfinimide products, but also enables bioconjugation of proteins and DNA through biotin redox-activated chemical labeling technology. Taking advantage of the streptavidin capture and photo-release properties, Biotin Azide Plus effectively facilitates the isolation of lipid-derived electrophile-protein adducts, thus finding wide application in scientific research related to fields such as SKBR3 cancer^[1][2].

Bioconjugation Application of Biotin Azide Plus - Protein Labeling^[1]:
Substrate: Biotinylated Trastuzumab (HY-P9907) (containing Avi-tag).
Conditions: 50 μM protein + 5-fold molar excess of reagent, reaction in PBS at room temperature for 1 h.
Bioconjugation Application of Biotin Azide Plus-DNA Labeling^[1]:
Substrate: 5'-biotinylated single-stranded DNA.
Conditions: 5-fold molar excess of reagent, reaction in aqueous solution at room temperature for 1 h.
Results: Azide handles are efficiently introduced, while no labeling is observed on non-biotinylated DNA.
Biotin Azide Plus exhibits photocleavable properties, and quantitative cleavage is achieved upon irradiation with low-intensity 365 nm ultraviolet light (4 W handheld UV lamp) at room temperature for 1 h^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

582.72

C₂₄H₄₂N₁₀O₅S

2669097-31-6

Solid

White to off-white

[H][C@]12NC(N[C@]1(CS[C@H]2CCCCC(NCCOCCOCCOCCN3C=C(N=N3)CNCCCN=[N+]=[N-])=O)[H])=O

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Cotton AD, et al. Biotin as a Reactive Handle to Selectively Label Proteins and DNA with Small Molecules. ACS Chem Biol. 2022;17(12):3270-3275.  [Content Brief]

  [2]. Kim H Y H, et al. An azido-biotin reagent for use in the isolation of protein adducts of lipid-derived electrophiles by streptavidin catch and photorelease[J]. Molecular & Cellular Proteomics, 2009, 8(9): 2080-2089.