Biotin Azide Plus
Based on 1 Customer Validation
Biotin Azide Plus is an oxazolidine reagent that integrates azide-biotin click chemistry and a photocleavable linker arm. Biotin Azide Plus not only reacts with biotin thioether to form stable sulfinimide products, but also enables bioconjugation of proteins and DNA through biotin redox-activated chemical labeling technology. Taking advantage of the streptavidin capture and photo-release properties, Biotin Azide Plus effectively facilitates the isolation of lipid-derived electrophile-protein adducts, thus finding wide application in scientific research related to fields such as SKBR3 cancer.
For research use only. We do not sell to patients.
- Purity: 98.38%
- CAS No.: 2669097-31-6
- Formula: C24H42N10O5S
- Molecular Weight:582.72
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Bioconjugation Application of Biotin Azide Plus - Protein Labeling[1]:
Substrate: Biotinylated Trastuzumab (HY-P9907) (containing Avi-tag).
Conditions: 50 μM protein + 5-fold molar excess of reagent, reaction in PBS at room temperature for 1 h.
Bioconjugation Application of Biotin Azide Plus-DNA Labeling[1]:
Substrate: 5'-biotinylated single-stranded DNA.
Conditions: 5-fold molar excess of reagent, reaction in aqueous solution at room temperature for 1 h.
Results: Azide handles are efficiently introduced, while no labeling is observed on non-biotinylated DNA.
Biotin Azide Plus exhibits photocleavable properties, and quantitative cleavage is achieved upon irradiation with low-intensity 365 nm ultraviolet light (4 W handheld UV lamp) at room temperature for 1 h[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2669097-31-6
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Appearance Solid
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Molecular Weight 582.72
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Formula C24H42N10O5S
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Color White to off-white
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SMILES
[H][C@]12NC(N[C@]1(CS[C@H]2CCCCC(NCCOCCOCCOCCN3C=C(N=N3)CNCCCN=[N+]=[N-])=O)[H])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 5.83 mg/mL (10.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7161 mL | 8.5805 mL | 17.1609 mL | 42.9023 mL |
| 5 mM | 0.3432 mL | 1.7161 mL | 3.4322 mL | 8.5805 mL |
Biotin Azide Plus Related Classifications
HY-W591393 Related Classifications
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