Biotin-XX hydrazide
Based on 1 Customer Validation
Biotin-XX hydrazide (Biotin-(AC5)2-hydrazide) is a carbonyl-reactive biotinylation reagent which contains two aminohexanoic acid spacers. Biotin-XX hydrazide has higher efficiency of avidin-binding.
For research use only. We do not sell to patients.
- Purity: 99.01%
- CAS No.: 211237-33-1
- Formula: C22H40N6O4S
- Molecular Weight:484.66
-
Storage:
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Biological Activity
Biotin-XX hydrazide contains two aminohexanoic acid spacers which greatly enhances the efficiency of formation of the complex between the biotinylated antibody (or other biotinylated protein) and the avidin–probe conjugate, where the probe can be a fluorochrome or an enzyme[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 211237-33-1
-
Appearance Solid
-
Molecular Weight 484.66
-
Formula C22H40N6O4S
-
Color White to off-white
-
SMILES
O=C(CCCCCNC(CCCCCNC(CCCC[C@@H]1SC[C@@]([H])([C@@]1(N2)[H])NC2=O)=O)=O)NN
-
Synonyms
Biotin-(AC5)2-hydrazide
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 5 mg/mL (10.32 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (266 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0633 mL | 10.3165 mL | 20.6330 mL | 51.5826 mL |
| 5 mM | 0.4127 mL | 2.0633 mL | 4.1266 mL | 10.3165 mL | |
| 10 mM | 0.2063 mL | 1.0317 mL | 2.0633 mL | 5.1583 mL |