BODIPY FL C5
Based on 1 Customer Validation
BODIPY FL C5 is a green fluorescent fatty acid. BODIPY FL C5 can be used as a precursor for the synthesis of various fluorescent phospholipids. BODIPY FL C5 is relatively insensitive to the environment and fluoresces in both water-soluble and lipid environments.
For research use only. We do not sell to patients.
- Purity: 99.59%
- CAS No.: 217075-24-6
- Formula: C16H19BF2N2O2
- Molecular Weight:320.14
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
A. Enzyme assays[1]:
1. An aliquot of phospholipid was placed in a 1×1-cm fluorescence cuvette and buffer (50 mM Tris, pH 8, 100 mM NaCl, 1 mM CaCl2) was added to a total volume of 1.3 mL. The temperature was allowed to equilibrate at 35°C with stirring for several minutes and the background emission was recorded (the background rate was undetectable). Enzyme (1-10 μL) was added and the emission was recorded.
2. An aliquot of phospholipid (78 μL of 0.05 mM substrate/0.5 mM DTPM) was placed in a fluorescence cuvette and buffer (50 mM Tris, pH 8, 100 mM NaCl, 1 mM CaCl2, 30% glycerol) was added to a total volume of 1.3 mL. The temperature was allowed to equilibrate at 35°C with stirring for several minutes and the background emission was recorded. Enzyme (1-10 μL) was added with extra mixing of the viscous solution, and
the emission was recorded.
3. A measured amount of BODIPYFL-C5 (in the case of PBPEC6DNP and MBPEDNP) or BODIPY-FL-C5-lyso-PAF (in the case of BC11-DNPC8-PC) was added to the phospholipid substrate solutions and the increase in emission was recorded. The factor, picomoles of BODIPY product/intensity unit increase, was then used to convert emission increase/sec to picomoles of product/second in the assays.
B. Zebrafish in vitro assay[1]:
1. Embryos were placed in 0.15 mL of embryo medium (EM: 13.7 mM NaCl, 0.537 mM KCl, 0.025 mM Na2HPO4, 0.044 mM KH2PO4, 1.30 mM CaCl2, 1 mM MgSO4, 4.2 mM NaHCO3, pH 7.2) .
2. Containing 150-200 ng of a fluorescent phospholipid substrate, and sonicated for 2-5 s.
3. After 1 h at 37°C, reactions were stopped by the addition of 0.45 mL of chloroform: methanol (2:1), mixed, and centrifuged (30 s, 16,000g) .
4. The aqueous fraction was discarded and an aliquot of organic fraction was loaded on thin-layer chromatography plates.
5. Plates were developed in toluene: diethyl ether: ethanol: acetic acid (50:40:2:0.2) and quantified using a laser scanner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 217075-24-6
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Appearance Solid
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Molecular Weight 320.14
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Formula C16H19BF2N2O2
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Color Orange to red
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SMILES
[F-][B+3]([F-])([N-]12)[N]3=C(C)C=C(C)C3=CC1=CC=C2CCCCC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (312.36 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1236 mL | 15.6182 mL | 31.2363 mL | 78.0908 mL |
| 5 mM | 0.6247 mL | 3.1236 mL | 6.2473 mL | 15.6182 mL | |
| 10 mM | 0.3124 mL | 1.5618 mL | 3.1236 mL | 7.8091 mL | |
| 15 mM | 0.2082 mL | 1.0412 mL | 2.0824 mL | 5.2061 mL | |
| 20 mM | 0.1562 mL | 0.7809 mL | 1.5618 mL | 3.9045 mL | |
| 25 mM | 0.1249 mL | 0.6247 mL | 1.2495 mL | 3.1236 mL | |
| 30 mM | 0.1041 mL | 0.5206 mL | 1.0412 mL | 2.6030 mL | |
| 40 mM | 0.0781 mL | 0.3905 mL | 0.7809 mL | 1.9523 mL | |
| 50 mM | 0.0625 mL | 0.3124 mL | 0.6247 mL | 1.5618 mL | |
| 60 mM | 0.0521 mL | 0.2603 mL | 0.5206 mL | 1.3015 mL | |
| 80 mM | 0.0390 mL | 0.1952 mL | 0.3905 mL | 0.9761 mL | |
| 100 mM | 0.0312 mL | 0.1562 mL | 0.3124 mL | 0.7809 mL |
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