1. Dye Reagents
  2. BODIPY FL Thapsigargin

BODIPY FL Thapsigargin 

Cat. No.: HY-D1608
Handling Instructions

BODIPY FL Thapsigargin is a potent green fluorescent dye. BODIPY FL Thapsigargin inhibits intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum. BODIPY FL Thapsigargin used for investigation of thapsigargin binding sites in live cells.

For research use only. We do not sell to patients.

BODIPY FL Thapsigargin Chemical Structure

BODIPY FL Thapsigargin Chemical Structure

CAS No. : 216571-99-2

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Description

BODIPY FL Thapsigargin is a potent green fluorescent dye. BODIPY FL Thapsigargin inhibits intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum. BODIPY FL Thapsigargin used for investigation of thapsigargin binding sites in live cells[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL Thapsigargin[1]:
1. Purified parasites are fixed with 1% formaldehyde in PBS (pH 8) for 1 h at room temperature and allows to adhere to poly-L-lysine-coated glass slides for 20 min Parasites are then permeated with 0.1% Triton X-100 for 5 min and blocked with 3% bovine serum albumin in PBS for 1 h.
2. Parasites were incubated at room temperature with a 1:100 dilution of the primary polyclonal antibody H-300 for 1 h, followed by a 1:100 dilution of Texa Red-coupled goat anti-rabbit IgG secondary antibody, and with 0.25 µM BODIPY FL TG for 5 min.
3. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

876.36

Formula

C44H57B3F2N2O122-

CAS No.
SMILES

O=C(/C(C)=C/C)O[[email protected]]1C(C)=C2[[email protected]@H]3OC([[email protected]@](C)(O)[[email protected]]3([[email protected]](C[[email protected]@](C)(C2[[email protected]@H]1OC(CCCCCCC)=O)OC(C)=O)OC(CCC4=CC=C5C=C6C(C)=CC(C)=[N]6B([F-])([F-])([N-]54)=B[B+])=O)O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Product Name:
BODIPY FL Thapsigargin
Cat. No.:
HY-D1608
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