BODIPY FL Thapsigargin 

BODIPY FL Thapsigargin is a potent green fluorescent dye. BODIPY FL Thapsigargin inhibits intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum. BODIPY FL Thapsigargin used for investigation of thapsigargin binding sites in live cells^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL Thapsigargin^[1]:
1. Purified parasites are fixed with 1% formaldehyde in PBS (pH 8) for 1 h at room temperature and allows to adhere to poly-L-lysine-coated glass slides for 20 min Parasites are then permeated with 0.1% Triton X-100 for 5 min and blocked with 3% bovine serum albumin in PBS for 1 h.
2. Parasites were incubated at room temperature with a 1:100 dilution of the primary polyclonal antibody H-300 for 1 h, followed by a 1:100 dilution of Texa Red-coupled goat anti-rabbit IgG secondary antibody, and with 0.25 μM BODIPY FL TG for 5 min.
3. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

876.36

C₄₄H₅₇B₃F₂N₂O₁₂^2-

216571-99-2

512

501

O=C(/C(C)=C/C)O[C@H]1C(C)=C2[C@@H]3OC([C@@](C)(O)[C@]3([C@H](C[C@@](C)(C2[C@@H]1OC(CCCCCCC)=O)OC(C)=O)OC(CCC4=CC=C5C=C6C(C)=CC(C)=[N]6B([F-])([F-])([N-]54)=B[B+])=O)O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Pérez-Gordones MC, et, al. Presence of a thapsigargin-sensitive calcium pump in Trypanosoma evansi: Immunological, physiological, molecular and structural evidences. Exp Parasitol. 2015 Dec;159:107-17.  [Content Brief]

  [2]. Urbina DC, et, al. The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana. Biol Res. 2006;39(2):289-96.  [Content Brief]