BODIPY FL Thapsigargin
BODIPY FL Thapsigargin is a potent green fluorescent dye. BODIPY FL Thapsigargin inhibits intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum. BODIPY FL Thapsigargin used for investigation of thapsigargin binding sites in live cells.
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- CAS No.: 216571-99-2
- Formule: C44H57BF2N2O12
- Masse moléculaire:854.74
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
BODIPY FL Thapsigargin[1]:
1. Purified parasites are fixed with 1% formaldehyde in PBS (pH 8) for 1 h at room temperature and allows to adhere to poly-L-lysine-coated glass slides for 20 min Parasites are then permeated with 0.1% Triton X-100 for 5 min and blocked with 3% bovine serum albumin in PBS for 1 h.
2. Parasites were incubated at room temperature with a 1:100 dilution of the primary polyclonal antibody H-300 for 1 h, followed by a 1:100 dilution of Texa Red-coupled goat anti-rabbit IgG secondary antibody, and with 0.25 μM BODIPY FL TG for 5 min.
3. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
512
Excitation (Ex)
501
Chemical Information
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CAS No. 216571-99-2
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Masse moléculaire 854.74
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Formule C44H57BF2N2O12
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SMILES
CCCCCCCC(O[C@@H]1[C@@H](OC(/C(C)=C\C)=O)C(C)=C2[C@@]1([H])[C@@](C)(OC(C)=O)C[C@H](OC(CCC3=CC=C(C=C(C(C)=C4)[N]5=C4C)[N-]3[B+3]5([F-])[F-])=O)[C@@]([C@@]6(O)C)(O)[C@@]2([H])OC6=O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1]. Pérez-Gordones MC, et, al. Presence of a thapsigargin-sensitive calcium pump in Trypanosoma evansi: Immunological, physiological, molecular and structural evidences. Exp Parasitol. 2015 Dec;159:107-17. [Content Brief]
[2]. Urbina DC, et, al. The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana. Biol Res. 2006;39(2):289-96. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BODIPY FL Thapsigargin Related Classifications
HY-D1608 Related Classifications
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