2. Immunology/Inflammation Apoptosis
  3. Macrophage migration inhibitory factor (MIF) Apoptosis
  4. BTZO-1

BTZO-1 binds to Macrophage migration inhibitory factor (MIF) with a Kd value of 68.6 nM, and its binding requires the N-terminal Pro1. BTZO-1 can activate antioxidant response element (ARE)-mediated gene expression and suppress oxidative stress-induced cardiomyocyte apoptosis in vitro.

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BTZO-1 Chemical Structure

BTZO-1 Chemical Structure

CAS No. : 99420-15-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 112 In-stock
Solution
10 mM * 1 mL in DMSO USD 112 In-stock
Solid
5 mg USD 101 In-stock
10 mg USD 195 In-stock
25 mg USD 395 In-stock
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Based on 1 publication(s) in Google Scholar

BTZO-1 Related Antibodies

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Description

BTZO-1 binds to Macrophage migration inhibitory factor (MIF) with a Kd value of 68.6 nM, and its binding requires the N-terminal Pro1. BTZO-1 can activate antioxidant response element (ARE)-mediated gene expression and suppress oxidative stress-induced cardiomyocyte apoptosis in vitro[1].

IC50 & Target

Kd:68.6 nM (Macrophage migration inhibitory factor)[1]
In Vitro

BTZO-1 (0-330 nM; 24 hours) dose-dependently suppressed serum deprivation-induced cardiomyocyte death; the minimum effective concentration (MEC1.5), the concentration of a sample necessary to give a 50% increase in cell viability, is 16 nM[1].
BTZO-1 (0.37 μM; 21 hours) upregulates mRNA protein levels of GST Ya and HO-1 in cardiomyocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BTZO-1 Related Antibodies

Cell Viability Assay[1]

Cell Line: Primary Neonatal Rat Cardiomyocytes
Concentration: 0-330 nM
Incubation Time: 24 hours
Result: Suppressed serum deprivation or DOX-induced cell death.
Molecular Weight

240.28

Formula

C13H8N2OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N=C(C2=NC=CC=C2)SC3=CC=CC=C13
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (34.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1618 mL 20.8091 mL 41.6181 mL
5 mM 0.8324 mL 4.1618 mL 8.3236 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1618 mL 20.8091 mL 41.6181 mL 104.0453 mL
5 mM 0.8324 mL 4.1618 mL 8.3236 mL 20.8091 mL
10 mM 0.4162 mL 2.0809 mL 4.1618 mL 10.4045 mL
15 mM 0.2775 mL 1.3873 mL 2.7745 mL 6.9364 mL
20 mM 0.2081 mL 1.0405 mL 2.0809 mL 5.2023 mL
25 mM 0.1665 mL 0.8324 mL 1.6647 mL 4.1618 mL
30 mM 0.1387 mL 0.6936 mL 1.3873 mL 3.4682 mL
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BTZO-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BTZO-199420-15-2BTZO1BTZO 1Macrophage migration inhibitory factor (MIF)ApoptosisCardiomyocyteoxidativestressInhibitorinhibitorinhibit

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