3-Nitro-L-tyrosine
Based on 1 Customer Validation
3-Nitro-L-tyrosine serves as a biomarker of oxidative stress. 3-Nitro-L-tyrosine attenuates the pressor and vasoconstrictive effects of angiotensin II by inhibiting the α1-adrenergic receptor-mediated signaling pathway, and participates in hemodynamic regulation under pathological conditions such as inflammation and ischemia. 3-Nitro-L-tyrosine can be used in studies related to atherosclerosis, ischemia-reperfusion and sepsis.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 621-44-3
- Formula: C9H10N2O5
- Molecular Weight:226.19
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Human Endogenous Metabolite |
3-Nitro-L-tyrosine can be found in the brain as a product of superoxide and nitric oxide, and serves as a marker for oxidative stress induced by reactive oxygen species and nitric oxide[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (male, 250-350 g, pentobarbital-anesthetized, surgically implanted with hemodynamic monitoring catheters and Doppler flow probes)[2]
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Dosage:2.5 μmol/kg
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Administration:i.v.; single bolus
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Result:Produced a significant 36% increase in baseline hindquarter vascular resistance at 120-180 min post-treatment.
Diminished the dose-dependent pressor responses to angiotensin II at 120-180 min post-treatment.
Significantly reduced the dose-dependent vasoconstrictor effects of angiotensin II in renal and mesenteric beds at 30-60 min post-treatment, with further attenuation evident at 120-180 min post-treatment.
Did not alter the minor effects of angiotensin II on hindquarter vascular resistance.
Chemical Information
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CAS No. 621-44-3
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Appearance Solid
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Molecular Weight 226.19
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Formula C9H10N2O5
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Color Light yellow to yellow
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SMILES
N[C@@H](CC1=CC([N+]([O-])=O)=C(C=C1)O)C(O)=O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : 4.76 mg/mL (21.04 mM; ultrasonic and adjust pH to 2 with 1 M HCl)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (287 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Maruyama W, et al. Identification of 3-nitro-L-tyrosine, a product of nitric oxide and superoxide, as an indicator of oxidative stress in the human brain. J Chromatogr B Biomed Appl. 1996 Feb 9;676(1):153-8. [Content Brief]
[2]. Kooy NW, et al. The peroxynitrite product 3-nitro-L-tyrosine attenuates the hemodynamic responses to angiotensin II in vivo. Eur J Pharmacol. 1996 Nov 14;315(2):165-70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.4211 mL | 22.1053 mL | 44.2106 mL | 110.5265 mL |
| 5 mM | 0.8842 mL | 4.4211 mL | 8.8421 mL | 22.1053 mL | |
| 10 mM | 0.4421 mL | 2.2105 mL | 4.4211 mL | 11.0527 mL | |
| 15 mM | 0.2947 mL | 1.4737 mL | 2.9474 mL | 7.3684 mL | |
| 20 mM | 0.2211 mL | 1.1053 mL | 2.2105 mL | 5.5263 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
3-Nitro-L-tyrosine Related Classifications
HY-113248 Related Classifications
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