NAI-N3
Based on 5 publication(s) in Google Scholar
NAI-N3 is a RNA acylation reagent that enables RNA purification. NAI-N3 is a dual-function SHAPE (selective 2-hydroxyl acylation and profiling experiment) probe (RNA structure probe and enrichment). It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity: 99.78%
- CAS No.: 1612756-29-2
- Formula: C10H8N6O
- Molecular Weight:228.21
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NAI-N3
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Biological Activity
Living cells are treated with the icSHAPE (in vivo click selective 2-hydroxyl acylation and profiling experiment) chemical NAI-N3 followed by selective chemical enrichment of NAI-N3-modified RNA, which provides an improved signal-to-noise ratio compared with similar methods leveraging deep sequencing[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1612756-29-2
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Appearance Solid
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Molecular Weight 228.21
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Formula C10H8N6O
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Color White to light yellow
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SMILES
O=C(C1=CC=CN=C1CN=[N+]=[N-])N2C=CN=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
2024 Jan;42(1):119-131. PMID: 37037902 -
Nat Commun
2024 Mar 18;15(1):2425. PMID: 38499544 -
Nucleic Acids Res
Conserved intronic secondary structures with concealed branch sites regulate alternative splicing of poison exons. [Abstract]2024 Jun 10;52(10):6002-6016. PMID: 38499485 -
Nucleic Acids Res
Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. [Abstract]2019 Dec 16;47(22):e145. PMID: 31584096 -
Solvent & Solubility
DMSO : 100 mg/mL (438.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 16.67 mg/mL (73.05 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (545 KB)
- English - EN (545 KB)
- Français - FR (545 KB)
- Deutsch - DE (545 KB)
- Norwegian - NO (545 KB)
- Español - ES (545 KB)
- Swedish - SV (545 KB)
- Italian - IT (545 KB)
- Korean - KR (545 KB)
- Portuguese - PT (545 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.3819 mL | 21.9096 mL | 43.8193 mL | 109.5482 mL |
| 5 mM | 0.8764 mL | 4.3819 mL | 8.7639 mL | 21.9096 mL | |
| 10 mM | 0.4382 mL | 2.1910 mL | 4.3819 mL | 10.9548 mL | |
| 15 mM | 0.2921 mL | 1.4606 mL | 2.9213 mL | 7.3032 mL | |
| 20 mM | 0.2191 mL | 1.0955 mL | 2.1910 mL | 5.4774 mL | |
| 25 mM | 0.1753 mL | 0.8764 mL | 1.7528 mL | 4.3819 mL | |
| 30 mM | 0.1461 mL | 0.7303 mL | 1.4606 mL | 3.6516 mL | |
| 40 mM | 0.1095 mL | 0.5477 mL | 1.0955 mL | 2.7387 mL | |
| 50 mM | 0.0876 mL | 0.4382 mL | 0.8764 mL | 2.1910 mL | |
| 60 mM | 0.0730 mL | 0.3652 mL | 0.7303 mL | 1.8258 mL | |
| DMSO | 80 mM | 0.0548 mL | 0.2739 mL | 0.5477 mL | 1.3694 mL |
| 100 mM | 0.0438 mL | 0.2191 mL | 0.4382 mL | 1.0955 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
NAI-N3 Related Classifications
HY-103006 Related Classifications
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