CRANAD-2
Based on 1 Customer Validation
CRANAD-2 is a specific near-infrared (NIR) fluorescent probe for detecting Aβ plaques (unbound to Aβ: Ex=640 nm; Em=805 nm; after binding: Em=715 nm). CRANAD-2 penetrates the blood-brain barrier and has high affinity for Aβ aggregates, with a Kd value of 38 nM. CRANAD-2 is applicable to research related to Alzheimer's disease and neurodegenerative diseases.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 1193447-34-5
- Formula: C23H25BF2N2O2
- Molecular Weight:410.26
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
CRANAD-2 (0-1500 nM; 1 h) binds to preformed ΔN amyloid fibrils with a dissociation constant of 55 ± 6 nM[1].
CRANAD-2 (35 nM) enables SMLM super-resolution imaging of preformed ΔN amyloid fibrils at a concentration of 35 nM, with a resolution of 45-55 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:transgenic arcAβ mice (overexpressing human APP695 with Swedish and Arctic mutations, both sexes, 18-24 months-of-age); non-transgenic littermates (both sexes, 18-24 months-of-age)[2]
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Dosage:2.0 mg/kg
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Administration:i.v.; single dose
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Result:Exhibited higher cortical retention in arcAβ mice compared to non-transgenic littermates at 60-120 minutes post-injection; Co-localized with anti-Aβ antibodies in parenchymal and vessel-associated Aβ deposits in arcAβ mouse brain sections.
Showed no detectable signal in non-transgenic littermate cortex sections.
Chemical Information
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CAS No. 1193447-34-5
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Appearance Solid
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Molecular Weight 410.26
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Formula C23H25BF2N2O2
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Color Brown to black
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SMILES
CN(C)C(C=C1)=CC=C1/C=C/C2=O[B+3]([F-])([F-])O=C(/C=C/C3=CC=C(N(C)C)C=C3)[CH-]2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 2.08 mg/mL (5.07 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
DMF : 2 mg/mL (4.87 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Torra J, et al. Versatile Near-Infrared Super-Resolution Imaging of Amyloid Fibrils with the Fluorogenic Probe CRANAD-2. Chemistry. 2022;28(19):e202200026. [Content Brief]
[2]. Ni R, et al. In-vitro and in-vivo characterization of CRANAD-2 for multi-spectral optoacoustic tomography and fluorescence imaging of amyloid-beta deposits in Alzheimer mice. Photoacoustics. 2021 Jul 15;23:100285. [Content Brief]
[3]. Ran C, et al. Design, synthesis, and testing of difluoroboron-derivatized curcumins as near-infrared probes for in vivo detection of amyloid-beta deposits. J Am Chem Soc. 2009 Oct 28;131(42):15257-61. [Content Brief]
[4]. Ran C, et al. Non-conjugated small molecule FRET for differentiating monomers from higher molecular weight amyloid beta species. PLoS One. 2011 Apr 29;6(4):e19362. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMF / DMSO | 1 mM | 2.4375 mL | 12.1874 mL | 24.3748 mL | 60.9370 mL |
| DMSO | 5 mM | 0.4875 mL | 2.4375 mL | 4.8750 mL | 12.1874 mL |
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