2-5A pentasodium solution (100 mM)
Based on 1 Customer Validation
2-5A (2′,5′-ApApA) pentasodium solution 100 mM is a high-affinity (Ka=0.04 nM) RNase L binder. 2-5A pentasodium solution 100 mM activates the nuclease activity of latent RNase L to cleave single-stranded RNA by inducing conformational changes and dimerization of latent RNase L, thereby mediating critical antiviral responses and enhancing interferon effects. 2-5A pentasodium solution 100 mM not only effectively inhibits Rauscher murine leukemia virus replication, reduces viremia and splenomegaly, but also enhances antileukemic efficacy when combined with amphotericin B and exhibits favorable high-dose tolerance. 2-5A pentasodium solution 100 mM is an important tool molecule for investigating the pathological mechanisms of viral infection, hereditary prostate cancer and leukemia.
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- Pureté: 90.0%
- Formule: C30H35N15Na5O25P5
- Masse moléculaire:1275.50
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Stockage:
Solution, -20°C, 2 years
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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RNASE L |
2-5A pentasodium solution 100 mM specifically binds to ankyrin repeat domains 2 and 4 of RNase L (K0.5=40 pM), induces its dimerization, and activates the RNA cleavage function of RNase L[1].
Endogenously produced 2-5A activates RNase L in interferon-treated mouse L fibroblasts infected with encephalomyocarditis virus, thereby inducing ribosomal RNA cleavage[1].
2-5A pentasodium solution 100 mM activates RNase L and induces the expression of the MIC-1 gene in mammalian cells via JNK- and ERK-dependent pathways[1].
2-5A pentasodium solution 100 mM activates RNase L and induces caspase-dependent apoptosis in mammalian cells via the JNK-mediated pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Synthetic (2’-5’)ApApA (100 μg-1 mg/mouse; i.p.; daily for 5 days or 15 total doses) exhibits dose-dependent antileukaemic activity (up to 26% splenomegaly inhibition) and potent antiviral activity (72% viraemia suppression, >95% RMLV replication inhibition) in RMLV-infected BALB/c male mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (male, RMLV-induced leukaemia)[2]
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Dosage:100 μg/mouse (15 doses + Amphotericin B);
0.5 mg/mouse (5 doses + Amphotericin B);
1 mg/mouse (5 doses; 5 doses + Amphotericin B; 15 doses); -
Administration:i.p.; daily for 5 consecutive days (days 1-5 post-infection); every 3 days for 15 total doses (days 1-5, 8-12, 15-19 post-infection)
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Result:Reduced average spleen weight by 17% (splenomegaly inhibition) and achieved >95% inhibition of RMLV replication in transformed spleen cells.
Reduced average spleen weight by 26% (splenomegaly inhibition, P < 0.05).
Reduced average spleen weight by 23% (splenomegaly inhibition, P < 0.1).
Reduced average spleen weight by 20% (splenomegaly inhibition).
Reduced average spleen weight by 17% (splenomegaly inhibition).
Reduced average spleen weight by 17% (splenomegaly inhibition) and suppressed viraemia by 72% (measured via serum reverse transcriptase activity).
Chemical Information
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Appearance Liquid
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Masse moléculaire 1275.50
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Formule C30H35N15Na5O25P5
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Color Colorless to light yellow
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SMILES
NC1=NC=NC2=C1N=CN2[C@H]3[C@H](OP(OC[C@@H](O[C@H]([C@@H]4OP(OC[C@@H](O[C@H]([C@@H]5O)N6C=NC7=C6N=CN=C7N)[C@H]5O)(O[Na])=O)N8C=NC9=C8N=CN=C9N)[C@H]4O)(O[Na])=O)[C@H](O)[C@@H](COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)O3
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Synonyms
2′,5′-ApApA pentasodium; 2′,5′-trioligoadenylate pentasodium; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine pentasodium
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Solution, -20°C, 2 years
Pureté et documentation
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Fiche technique (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2-5A pentasodium solution (100 mM) Related Classifications
HY-173189A Related Classifications
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- 2-5A pentasodium solution (100 mM)
- 2′,5′-ApApA pentasodium
- 2′,5′-trioligoadenylate pentasodium
- 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine pentasodium
- RSV
- DNA/RNA Synthesis
- BALB/c mice
- mouse cells
- viral infections
- mouse L fibroblasts
- mammalian cells
- interferon
- RNase L
- hereditary prostate cancer
- Rauscher murine leukaemia virus
- encephalomyocarditis virus
- Inhibitor
- inhibitor
- inhibit