2. Cell Cycle/DNA Damage Anti-infection
  3. DNA/RNA Synthesis RSV
  4. 2-5A

2-5A  (Synonyms: 2′,5′-ApApA; 2′,5′-trioligoadenylate; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine)

Cat. No.: HY-173189
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2-5A is a RNase L activator, signal mediator and innate immune second messenger. 2-5A induces the expression of interferons and interferon-stimulated genes in recipient cells in a RNase L-dependent manner. 2-5A inhibits tumor growth in mouse models via paracrine RNase L activation derived from cancer cells. 2-5A can be used in studies related to viral infection and cancer.

It is advisable to consider the salt form (2-5A TEA) that retains the same biological activity.

For research use only. We do not sell to patients.

2-5A

2-5A Chemical Structure

CAS No. : 65954-93-0

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2-5A Related Antibodies

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Description

2-5A is a RNase L activator, signal mediator and innate immune second messenger. 2-5A induces the expression of interferons and interferon-stimulated genes in recipient cells in a RNase L-dependent manner. 2-5A inhibits tumor growth in mouse models via paracrine RNase L activation derived from cancer cells. 2-5A can be used in studies related to viral infection and cancer[1].

IC50 & Target

RNASE L

 

In Vitro

2-5A induces paracrine RNase L activation in co-cultured A549 human lung adenocarcinoma epithelial cell lines, with activation triggered by poly(I:C) treatment or expression of the constitutively active OAS1A76V mutant[1].
2-5A is exported extracellularly by poly(I:C)-stimulated MC38 murine colon adenocarcinoma cells via the ABCC10 and ABCC1 transporters[1].
Trimeric 2-5A is taken up by HEK293T human embryonic kidney cells and BJ1 human foreskin fibroblasts to induce RNase L-dependent IFN/ISG activation, with LRRC8A and P2X7R contributing to 2-5A import in HEK293T cells[1].
2-5A export via gap junctions (A549 cells) or ABCC10 (MC38 cells) mediates paracrine OAS-RNase L activation and antiviral immunity against VSV and SeV infection[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-5A Related Antibodies
In Vivo

Endogenously produced 2-5A (Endogenously produced by implanted MC38 colon adenocarcinoma cells) released from MC38 colon adenocarcinoma cells restrains primary tumor growth in mice via activation of host RNase L, and this effect is dependent on 2-5A export through ABCC10[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

1165.59

Formula

C30H40N15O25P5
CAS No.
SMILES

NC1=NC=NC2=C1N=CN2[C@H]3[C@H](OP(O)(OC[C@@H](O[C@H]([C@@H]4OP(O)(OC[C@@H](O[C@H]([C@@H]5O)N6C=NC7=C6N=CN=C7N)[C@H]5O)=O)N8C=NC9=C8N=CN=C9N)[C@H]4O)=O)[C@H](O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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2-5A Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-5A65954-93-02′,5′-ApApA 2′,5′-trioligoadenylate 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosineDNA/RNA SynthesisRSVRespiratory syncytial virusOAS-RNase L pathwayA549HEK293TLRRC8ARNase LABCC1ABCC10MC38ENPP1P2X7RInhibitorinhibitorinhibit

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