2-5A pentasodium solution (100 mM)
Based on 1 Customer Validation
2-5A (2′,5′-ApApA) pentasodium solution 100 mM is a high-affinity (Ka=0.04 nM) RNase L binder. 2-5A pentasodium solution 100 mM activates the nuclease activity of latent RNase L to cleave single-stranded RNA by inducing conformational changes and dimerization of latent RNase L, thereby mediating critical antiviral responses and enhancing interferon effects. 2-5A pentasodium solution 100 mM not only effectively inhibits Rauscher murine leukemia virus replication, reduces viremia and splenomegaly, but also enhances antileukemic efficacy when combined with amphotericin B and exhibits favorable high-dose tolerance. 2-5A pentasodium solution 100 mM is an important tool molecule for investigating the pathological mechanisms of viral infection, hereditary prostate cancer and leukemia.
For research use only. We do not sell to patients.
- Purity: 90.0%
- Formula: C30H35N15Na5O25P5
- Molecular Weight:1275.50
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Storage:
Solution, -20°C, 2 years
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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RNASE L |
2-5A pentasodium solution 100 mM specifically binds to ankyrin repeat domains 2 and 4 of RNase L (K0.5=40 pM), induces its dimerization, and activates the RNA cleavage function of RNase L[1].
Endogenously produced 2-5A activates RNase L in interferon-treated mouse L fibroblasts infected with encephalomyocarditis virus, thereby inducing ribosomal RNA cleavage[1].
2-5A pentasodium solution 100 mM activates RNase L and induces the expression of the MIC-1 gene in mammalian cells via JNK- and ERK-dependent pathways[1].
2-5A pentasodium solution 100 mM activates RNase L and induces caspase-dependent apoptosis in mammalian cells via the JNK-mediated pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Synthetic (2’-5’)ApApA (100 μg-1 mg/mouse; i.p.; daily for 5 days or 15 total doses) exhibits dose-dependent antileukaemic activity (up to 26% splenomegaly inhibition) and potent antiviral activity (72% viraemia suppression, >95% RMLV replication inhibition) in RMLV-infected BALB/c male mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (male, RMLV-induced leukaemia)[2]
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Dosage:100 μg/mouse (15 doses + Amphotericin B);
0.5 mg/mouse (5 doses + Amphotericin B);
1 mg/mouse (5 doses; 5 doses + Amphotericin B; 15 doses); -
Administration:i.p.; daily for 5 consecutive days (days 1-5 post-infection); every 3 days for 15 total doses (days 1-5, 8-12, 15-19 post-infection)
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Result:Reduced average spleen weight by 17% (splenomegaly inhibition) and achieved >95% inhibition of RMLV replication in transformed spleen cells.
Reduced average spleen weight by 26% (splenomegaly inhibition, P < 0.05).
Reduced average spleen weight by 23% (splenomegaly inhibition, P < 0.1).
Reduced average spleen weight by 20% (splenomegaly inhibition).
Reduced average spleen weight by 17% (splenomegaly inhibition).
Reduced average spleen weight by 17% (splenomegaly inhibition) and suppressed viraemia by 72% (measured via serum reverse transcriptase activity).
Chemical Information
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Appearance Liquid
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Molecular Weight 1275.50
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Formula C30H35N15Na5O25P5
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Color Colorless to light yellow
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SMILES
NC1=NC=NC2=C1N=CN2[C@H]3[C@H](OP(OC[C@@H](O[C@H]([C@@H]4OP(OC[C@@H](O[C@H]([C@@H]5O)N6C=NC7=C6N=CN=C7N)[C@H]5O)(O[Na])=O)N8C=NC9=C8N=CN=C9N)[C@H]4O)(O[Na])=O)[C@H](O)[C@@H](COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)O3
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Synonyms
2′,5′-ApApA pentasodium; 2′,5′-trioligoadenylate pentasodium; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine pentasodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2-5A pentasodium solution (100 mM) Related Classifications
HY-173189A Related Classifications
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- 2-5A pentasodium solution (100 mM)
- 2′,5′-ApApA pentasodium
- 2′,5′-trioligoadenylate pentasodium
- 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine pentasodium
- RSV
- DNA/RNA Synthesis
- BALB/c mice
- mouse cells
- viral infections
- mouse L fibroblasts
- mammalian cells
- interferon
- RNase L
- hereditary prostate cancer
- Rauscher murine leukaemia virus
- encephalomyocarditis virus
- Inhibitor
- inhibitor
- inhibit