Cat. No.: HY-N0578

Apigenin 7-glucoside

Name: Apigenin 7-glucoside
Brand: MedChemExpress
SKU: HY-N0578
Price: 50.00 USD
Availability: InStock
Rating: 5 (8 reviews)

(Synonyms: Apigenin-7-O-β-D-glucopyranoside; Cosmosiin; Apigetrin)

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

Apigenin-7-glucoside (Apigenin-7-O-β-D-glucopyranoside) exhibits significant anti-proliferative and antioxidant activity and scavenges reactive oxygen species (ROS).

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Pureté : 98.98%
CAS No.: 578-74-5
Formule: C₂₁H₂₀O₁₀
Masse moléculaire:432.38

Stockage:

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Apigenin 7-glucoside Chemical Structure

CAS No.: 578-74-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (8)
Solvant et solubilité
- In Vitro
- In Vivo
Protocole (2)
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Apigenin-7-glucoside (Apigenin-7-O-β-D-glucopyranoside) exhibits significant anti-proliferative and antioxidant activity and scavenges reactive oxygen species (ROS)^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
Erythrocyte	IC₅₀	88.4 μM Compound: 12	Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs	[PMID: 12828473]
H9	EC₅₀	1.8 μg/mL Compound: 3	Inhibitory activity against HIV-1 IIIB replication in H9 cell line Inhibitory activity against HIV-1 IIIB replication in H9 cell line	10.1016/0960-894X(94)80015-4
H9	EC₅₀	61 μM Compound: 2	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
H9	IC₅₀	>100 μg/mL Compound: 3	Inhibitory activity against H9 cells uninfected with HIV-1 Inhibitory activity against H9 cells uninfected with HIV-1	10.1016/0960-894X(94)80015-4
H9	IC₅₀	115 μM Compound: 2	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
H9c2	EC₅₀	12.3 μM Compound: 6	Cytoprotective activity against doxorubicin-induced cytotoxicity in rat H9c2 cells assessed as cell viability after 24 hrs by MTT assay Cytoprotective activity against doxorubicin-induced cytotoxicity in rat H9c2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 20932762]
HeLa S3	IC₅₀	>10 μM Compound: 12	Cytotoxicity against human HeLa S3 cells assessed as cell viability incubated for 48 hrs by microscopic analysis Cytotoxicity against human HeLa S3 cells assessed as cell viability incubated for 48 hrs by microscopic analysis	[PMID: 35704432]
HepG2	IC₅₀	90.6 μM Compound: 5, Apigenin-7-glucoside	Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24974349]
HT-22	EC₅₀	>100 μM Compound: 11	Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay	[PMID: 32991171]
KB	ED₅₀	>100 μg/mL Compound: NSC-407303	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
Panel NCI-60 (60 carcinoma cell lines)	GI₅₀	76 nM Compound: Cosmosiin	Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program	[PMID: 15743190]
RAW264.7	CC₅₀	>100 μM Compound: 23	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 33667099]
RAW264.7	IC₅₀	>20 μM Compound: apigenin-7-O-beta-D-glucopyranoside	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitrite accumulation after 24 hrs by Griess reagent method Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitrite accumulation after 24 hrs by Griess reagent method	[PMID: 19778086]
RAW264.7	IC₅₀	>50 μM Compound: 8	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA	[PMID: 31747281]
RAW264.7	IC₅₀	53.7 μM Compound: 23	Inhibition of LPS induced NO production in mouse RAW264.7 cells measured after 24 hrs by Griess reagent based assay Inhibition of LPS induced NO production in mouse RAW264.7 cells measured after 24 hrs by Griess reagent based assay	[PMID: 33667099]

In Vitro

Apigenin 7-glucoside exhibits significant anti-proliferative activity against B16F10 melanoma cells after 24 and 48 h of incubation. Apigenin-7-glucoside provoks an increase of subG0/G1, S and G2/M phase cell proportion with a significant decrease of cell proportion in G0/G1 phases. Apigenin-7-glucoside enhances melanogenesis synthesis and tyrosinase activity of B16F10 melanoma cells^[1].
Apigenin 7-glucoside specifically induces the differentiation of CD34⁺ cells towards the erythroid lineage and inhibited the myeloid differentiation. Apigenin 7-glucoside has strong antioxidant activity against reactive oxygen species (ROS) in vitro in a concentration-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 578-74-5
Appearance Solid
Masse moléculaire 432.38
Formule C₂₁H₂₀O₁₀
Color Light yellow to yellow
SMILES

O=C(C=C(C1=CC=C(O)C=C1)OC2=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=C4)C2=C4O
Synonyms

Apigenin-7-O-β-D-glucopyranoside; Cosmosiin; Apigetrin
Structure Classification
- Flavonoids
- Flavones
- Phenols
- Polyphenols
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Publications (8)

All WBIHCCell Proliferation/Viability AssayFlow CytometryIF

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Journal Impact Factor

Adv Sci (Weinh)

SHMT2 Mediates Small-Molecule-Induced Alleviation of Alzheimer Pathology Via the 5'UTR-dependent ADAM10 Translation Initiation. [Abstract]

2024 Mar;11(11):e2305260. PMID: 38183387
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530
Ind Crops Prod

2025 Dec 11;239:122458.
Front Pharmacol

Anti-inflammatory and antioxidant effects of Pogostemon stellatus (Lour.) Kuntze via MAPK, NF-κB, and Nrf2 signaling pathways in LPS-activated RAW 264.7 macrophages. [Abstract]

2025 Sep 25:16:1679919. PMID: 41079749
JOR Spine

Apigetrin alleviates intervertebral disk degeneration by regulating nucleus pulposus cell autophagy. [Abstract]

2024 Apr 17;7(2):e1325. PMID: 38633661

Apigenin 7-glucoside purchased from MedChemExpress. Usage Cited in: JOR Spine. 2024 Apr 17;7(2):e1325. [Abstract]

Western blotting was used to detect the expression of anabolism-related protein (type II collagen), catabolism-related protein (MMP13), and inflammation-related protein (inducible nitric oxide synthase [iNOS]) in NPCs treated with 5 ng/mL IL-1β alone or in combination with varying concentrations of API (Apigenin 7-glucoside: 5, 10, 25, and 50 μM).

Apigenin 7-glucoside purchased from MedChemExpress. Usage Cited in: JOR Spine. 2024 Apr 17;7(2):e1325. [Abstract]

CCK-8 results show the cell viability of NPCs treated with different concentrations of API (Apigenin 7-glucoside: 0, 5, 10, 25, 50, and 100 μM).

Apigenin 7-glucoside purchased from MedChemExpress. Usage Cited in: JOR Spine. 2024 Apr 17;7(2):e1325. [Abstract]

API (Apigenin 7-glucoside: 50 μM). The expression of MMP9 and MMP13 was detected by immunofluorescence.
Clin Transl Oncol

Apigenin 7-glucoside reprograms tumor metabolism and enhances immunotherapy efficacy in colorectal cancer via DLX5. [Abstract]

2026 Feb 25. PMID: 41739401

Apigenin 7-glucoside purchased from MedChemExpress. Usage Cited in: Clin Transl Oncol. 2026 Feb 25. [Abstract]

Representative immunohistochemical staining of the endothelial cell markers CD31 and CD34 in tumor sections from the NC group and the A7G (Apigenin 7-glucoside: 20 mg/kg) treatment group. The area of positive staining was significantly reduced in the A7G group, indicating an inhibition of tumor angiogenesis.

Apigenin 7-glucoside purchased from MedChemExpress. Usage Cited in: Clin Transl Oncol. 2026 Feb 25. [Abstract]

Combination therapy with A7G (apigenin 7-glucoside; 20 mg/kg) and anti-PD-1 therapy increases tumor-infiltrating CD8⁺ T cells and reduces regulatory T cells in CT26 tumors. Tumor-infiltrating CD8⁺ T cells were analyzed by flow cytometry.
University of Belgrade

2019 Jul.

Solvant et solubilité

In Vitro:

DMSO : ≥ 100 mg/mL (231.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3128 mL

11.5639 mL

23.1278 mL

5 mM

0.4626 mL

2.3128 mL

4.6256 mL

10 mM

0.2313 mL

1.1564 mL

2.3128 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocole

All Cell Biology

ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (274 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Nasr Bouzaiene N et al. Effect of apigenin-7-glucoside, genkwanin and naringenin on tyrosinase activity and melanin synthesis in B16F10 melanoma cells. Life Sci. 2016 Jan 1;144:80-5. [Content Brief]

[2]. Samet I et al. Olive leaf components apigenin 7-glucoside and luteolin 7-glucoside direct human hematopoietic stem cell differentiation towards erythroid lineage. Differentiation. 2015 Jun;89(5):146-55. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3128 mL	11.5639 mL	23.1278 mL	57.8195 mL
	5 mM	0.4626 mL	2.3128 mL	4.6256 mL	11.5639 mL
	10 mM	0.2313 mL	1.1564 mL	2.3128 mL	5.7820 mL
	15 mM	0.1542 mL	0.7709 mL	1.5419 mL	3.8546 mL
	20 mM	0.1156 mL	0.5782 mL	1.1564 mL	2.8910 mL
	25 mM	0.0925 mL	0.4626 mL	0.9251 mL	2.3128 mL
	30 mM	0.0771 mL	0.3855 mL	0.7709 mL	1.9273 mL
	40 mM	0.0578 mL	0.2891 mL	0.5782 mL	1.4455 mL
	50 mM	0.0463 mL	0.2313 mL	0.4626 mL	1.1564 mL
	60 mM	0.0385 mL	0.1927 mL	0.3855 mL	0.9637 mL
	80 mM	0.0289 mL	0.1445 mL	0.2891 mL	0.7227 mL
	100 mM	0.0231 mL	0.1156 mL	0.2313 mL	0.5782 mL

Bioz

Keywords

Apigenin 7-glucoside

8 Cited Publications

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Apigenin 7-glucoside

Publications Citing Use of MedChemExpress (MCE) Apigenin 7-glucoside