BAI1
Based on 13 publication(s) in Google Scholar
BAI1 is a selective and allosteric inhibitor of BAX, an apoptosis regulator. BAI1 directly binds to BAX and allosterically inhibits BAX activation. BAI1 has the potential for the research of diseases mediated by BAX-dependent cell death.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.68%
- CAS No.: 335165-68-9
- Formule: C19H21Br2N3O
- Masse moléculaire:467.20
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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HY-50907
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VBIT-4VDAC1 Inhibitor -
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BH3I-1Bcl-2/Bcl-XL Inhibitor -
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Z-VAD-FMKPan-Caspase Inhibitor -
HY-19741
A-1331852Bcl-xL Inhibitor -
HY-118948
MSN-50Bax/Bak Inhibitor -
HY-120079
MSN-125Bax/Bak Oligomerization Inhibitor -
HY-15531R
Venetoclax (Standard)BCL-2 Inhibitor
Publications Citing Use of MedChemExpress (MCE) BAI1
More- Adv Funct Mater. 2023 Apr 28.
- Nat Commun. 2025 Aug 26;16(1):7936. [Abstract]
- Nat Commun. 2024 Dec 30;15(1):10806. [Abstract]
- Nat Commun. 2023 Nov 20;14(1):7547. [Abstract]
- Mater Today Bio. 2026 Apr 22:38:103132. [Abstract]
- Biochem Pharmacol. 2025 Jul 17:117177. [Abstract]
- Cell Mol Life Sci. 2025 Apr 7;82(1):142. [Abstract]
- Int Immunopharmacol. 2024 Oct 21;143(Pt 2):113424. [Abstract]
- Int J Mol Sci. 2023 May 11;24(10):8609. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2026 Jul 28;1873(1):168388.
- Ren Fail. 2024 Dec;46(2):2415519. [Abstract]
- Nat Prod Commun. 2025 Apr 29.
- University of Rijeka. 2021 Jun.
Customer Validation & Images
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IF
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Bio/Physico-chemical Assay
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Histological Imaging/Staining
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RT-PCR
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Flow Cytometry
Activité biologique
Description
IC50 & Target
[1]|
Bax |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HeLa | IC50 |
0.52 μM
Compound: 1
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In vitro inhibition of Bax regulated release of cytochrome C was determined using liposome channel assay
In vitro inhibition of Bax regulated release of cytochrome C was determined using liposome channel assay
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[PMID: 14521400] |
In Vitro
BAI1 selectively inhibits BAX-mediated apoptotic cell death[1].
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BAI1 shows inhibition of tBID (HY-100464)-induced BAX-mediated membrane permeabilization in a dose dependent manner with an IC50 of 3.3 μΜ[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Mouse MEF
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Concentration:0.3125 μM , 0.625 μM, 1 μM, 1.25 μM, 1.5 μM, 2.5 μM , 3 μM, 4 μM, 5 4 μM, 10 4 μM
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Incubation Time:8 hours
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Result:Inhibited cell death in BAX-dependent manner.
Chemical Information
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CAS No. 335165-68-9
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Appearance Solid
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Masse moléculaire 467.20
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Formule C19H21Br2N3O
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Color White to off-white
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SMILES
OC(CN1CCNCC1)CN2C3=C(C4=C2C=CC(Br)=C4)C=C(Br)C=C3
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (13)
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Journal Impact Factor
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Most Recent
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Nat Commun
A multifaceted strategy for intra- and extracellular nucleic acid regulation to alleviate intervertebral disc degeneration. [Abstract]2025 Aug 26;16(1):7936. PMID: 40858573
BAI1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 26;16(1):7936. [Abstract]
Immunofluorescence staining and quantification of BAX6A7 and dsDNA in aging NP cells treated with BAI1 (2.5 μM, 24 h), BNPM, or BNPMT.
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Nat Commun
2024 Dec 30;15(1):10806. PMID: 39737939 -
Nat Commun
2023 Nov 20;14(1):7547. PMID: 37985759 -
Mater Today Bio
2026 Apr 22:38:103132. PMID: 42111771 -
Biochem Pharmacol
Targeting STING alleviates pyroptosis of bladder epithelial cells and ameliorates bladder fibrosis in neurogenic bladder. [Abstract]2025 Jul 17:117177. PMID: 40683470 -
Cell Mol Life Sci
Dietary advanced glycation end-products exacerbate sarcopenia onset by activating apoptosis through PRMT1-mediated CRTC3 arginine methylation. [Abstract]2025 Apr 7;82(1):142. PMID: 40192801
BAI1 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Apr 7;82(1):142. [Abstract]
Levels of free and protein-bound CML were significantly increased in both the BAI1 (50 mg/kg, i.p.)-treated and HAGE groups compared to the LAGE group.
BAI1 purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Apr 7;82(1):142. [Abstract]
Histological analysis of skeletal muscle tissues demonstrated that BAI1 (50 mg/kg, i.p.) treatment significantly increased muscle fiber size and decreased connective tissue accumulation compared to the HAGE group.
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Int Immunopharmacol
BAX pores facilitate mitochondrial DNA release in wasp sting-induced acute kidney injury. [Abstract]2024 Oct 21;143(Pt 2):113424. PMID: 39437488 -
Int J Mol Sci
Mitochondrial Trafficking of MLKL, Bak/Bax, and Drp1 Is Mediated by RIP1 and ROS which Leads to Decreased Mitochondrial Membrane Integrity during the Hyperglycemic Shift to Necroptosis. [Abstract]2023 May 11;24(10):8609. PMID: 37239951 -
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Ren Fail
Piezo1 facilitates the initiation and progression of renal fibrosis by mediating cell apoptosis and mitochondrial dysfunction. [Abstract]2024 Dec;46(2):2415519. PMID: 39496543
BAI1 purchased from MedChemExpress. Usage Cited in: Ren Fail. 2024 Dec;46(2):2415519. [Abstract]
qPCR results indicated that Bax, Cytc, Caspase3, Cleaved-caspase3, KIM1, Fn and Collagen I expression were higher in the Yoda1 treatment group than in the DMSO group but lower in the Yoda1 + BAI1 (4 μM) group than in Yoda1 group.
BAI1 purchased from MedChemExpress. Usage Cited in: Ren Fail. 2024 Dec;46(2):2415519. [Abstract]
Flow cytometry scatter plot of JC1 staining after Yoda1 or/and BAI1 (4 μM) co-treatment, the FITC+ cells present the decreased of mitochondrial membrane potential.
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Solvant et solubilité
In Vitro:
DMSO : 18.46 mg/mL (39.51 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.87 mg/mL (4.00 mM); Clear solution
This protocol yields a clear solution of ≥ 1.87 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (18.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
Pureté et documentation
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Fiche technique (278 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1404 mL | 10.7021 mL | 21.4041 mL | 53.5103 mL |
| 5 mM | 0.4281 mL | 2.1404 mL | 4.2808 mL | 10.7021 mL | |
| 10 mM | 0.2140 mL | 1.0702 mL | 2.1404 mL | 5.3510 mL | |
| 15 mM | 0.1427 mL | 0.7135 mL | 1.4269 mL | 3.5674 mL | |
| 20 mM | 0.1070 mL | 0.5351 mL | 1.0702 mL | 2.6755 mL | |
| 25 mM | 0.0856 mL | 0.4281 mL | 0.8562 mL | 2.1404 mL | |
| 30 mM | 0.0713 mL | 0.3567 mL | 0.7135 mL | 1.7837 mL |
BAI1 Related Classifications
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