BTR-1
Based on 1 Customer Validation
BTR-1 is an active anti-cancer agent, causes S phase arrest, and affects DNA replication in leukemic cells. BTR-1 activates apoptosis and induces cell death.
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- Pureté: 99.96%
- CAS No.: 18331-34-5
- Formule: C12H11NOS2
- Masse moléculaire:249.35
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Apoptosis[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
6 μM
Compound: BTR-1
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Cytotoxicity against human CEM cells after 72 hrs by trypan blue assay
Cytotoxicity against human CEM cells after 72 hrs by trypan blue assay
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[PMID: 20832305] |
| CCRF-CEM | IC50 |
8 μM
Compound: BTR-1
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Cytotoxicity against human CEM cells after 48 hrs by trypan blue assay
Cytotoxicity against human CEM cells after 48 hrs by trypan blue assay
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[PMID: 20832305] |
BTR-1 (10, 50, 100, and 250 µM, 5 days) dose-dependently causes cytotoxicity in human leukemic cells, with IC50s of 8 and 6 µM at 48 and 72 h, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 18331-34-5
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Appearance Solid
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Masse moléculaire 249.35
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Formule C12H11NOS2
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Color Light yellow to yellow
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SMILES
S=C(N(CC)C/1=O)SC1=C/C2=CC=CC=C2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 25 mg/mL (100.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0104 mL | 20.0521 mL | 40.1043 mL | 100.2607 mL |
| 5 mM | 0.8021 mL | 4.0104 mL | 8.0209 mL | 20.0521 mL | |
| 10 mM | 0.4010 mL | 2.0052 mL | 4.0104 mL | 10.0261 mL | |
| 15 mM | 0.2674 mL | 1.3368 mL | 2.6736 mL | 6.6840 mL | |
| 20 mM | 0.2005 mL | 1.0026 mL | 2.0052 mL | 5.0130 mL | |
| 25 mM | 0.1604 mL | 0.8021 mL | 1.6042 mL | 4.0104 mL | |
| 30 mM | 0.1337 mL | 0.6684 mL | 1.3368 mL | 3.3420 mL | |
| 40 mM | 0.1003 mL | 0.5013 mL | 1.0026 mL | 2.5065 mL | |
| 50 mM | 0.0802 mL | 0.4010 mL | 0.8021 mL | 2.0052 mL | |
| 60 mM | 0.0668 mL | 0.3342 mL | 0.6684 mL | 1.6710 mL | |
| 80 mM | 0.0501 mL | 0.2507 mL | 0.5013 mL | 1.2533 mL | |
| 100 mM | 0.0401 mL | 0.2005 mL | 0.4010 mL | 1.0026 mL |
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