CS790AM
CS790AM (Coppersensor 790 acetoxymethyl ester) is a cell-permeable, Cu+-targeted near-infrared fluorescent probe (λabs=760 nm, λem=790 nm) applicable to live cells. CS790AM can cross lipophilic cell membranes, and is converted into negatively charged CS790 under the action of intracellular esterases to be retained, thus enabling highly sensitive, reversible "turn-on" detection of labile Cu+ pools in live cells and mice. CS790AM possesses excellent biocompatibility and selectivity, avoids interference from other metal ions, shows no obvious toxicity, and can be rapidly cleared. CS790AM allows long-term longitudinal monitoring of individual mice, visualizes copper levels in internal organs and isolated livers, and effectively evaluates abnormal copper accumulation in Wilson's disease models (Atp7b-/-) as well as dynamic changes after chelator treatment. CS790AM can be used for research on Wilson's disease and related copper metabolic disorders.
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- CAS No.: 1416808-87-1
- Formule: C56H70BrN3O9S3
- Masse moléculaire:1105.27
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
CS790AM, a Cy7-based fluorescent probe, produces a 17-fold fluorescence turn-on response upon binding to Cu+ in cell-free conditions[1].
CS790AM (2 μM; 15 min at 37 °C) reversibly detects fluctuations in labile copper levels in HEK 293T cells, with a significant increase in mean fluorescence intensity in copper-overloaded cells that is reversed by copper chelation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
CS790AM (0.1 mM; i.p.; single dose) detects aberrantly elevated labile Cu+ levels in Atp7b-/- Wilson disease model mice, producing significantly higher fluorescence signal than in wild-type mice at 30 minutes post-injection, with signal reducible by copper chelation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SKH-1 (hairless)[2]
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Dosage:0.1 mM
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Administration:i.p.; single dose
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Result:Reversed the ~60% fluorescence increase when co-administered with ATN-224 (HY-16074).
Showed significantly lower fluorescence in mice pretreated with ATN-224 alone compared to mice treated with CS790AM alone.
Confirmed higher fluorescence in copper-pretreated mice via ex vivo liver imaging, with total photon flux values significantly elevated compared to vehicle-pretreated controls.
Showed sustained elevated fluorescence in copper-pretreated mice relative to controls for up to 9 hours post-injection, with signal fully attenuated by 72 hours.
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Animal Model:C57BL/6 (7-11 weeks old, female; Wilson disease model via Atp7b gene inactivation); wild-type (7-11 weeks old, female)[2]
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Dosage:0.1 mM
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Administration:i.p.; single dose
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Result:Exhibited significantly higher in vivo fluorescence signal in Atp7b-/- mice than wild-type mice at 30 minutes post-injection.
Showed reduced fluorescence in ATN-224-pretreated Atp7b-/- mouse livers compared to vehicle-pretreated controls via ex vivo imaging, correlating with decreased copper levels in serum.
Chemical Information
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CAS No. 1416808-87-1
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Masse moléculaire 1105.27
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Formule C56H70BrN3O9S3
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SMILES
O=C(CC[N+]1=C(C(C)(C2=CC=CC=C21)C)/C=C/C(CCC/C3=C\C=C4N(C5=CC=CC=C5C\4(C)C)CCC(OCOC(C)=O)=O)=C3OC6=CC(SCCCSC)=C(C=C6)NCCCSC)OCOC(C)=O.[Br-]
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Synonyms
Coppersensor 790 acetoxymethyl ester
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[2]. Hirayama T, et al. Near-infrared fluorescent sensor for in vivo copper imaging in a murine Wilson disease model. Proc Natl Acad Sci U S A. 2012;109(7):2228-2233. [Content Brief]
[3]. Cotruvo JA Jr, et al. Synthetic fluorescent probes for studying copper in biological systems. Chem Soc Rev. 2015;44(13):4400-4414. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CS790AM Related Classifications
HY-W783351 Related Classifications
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