DDAO-C6

DDAO-C6 (10 μM; 30 min; 37 °C) shows a linear relationship between the fluorescence intensities and lipase concentrations (0-50 μg/mL), and products a red fluorescence signal in the presence of active lipase^[1].
DDAO-C6 hydrolysates have fluorescence properties, with excitation wavelength = 550-600 nm, emission spectrum = 630-700 nm^[2].

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Intestinal Fungi^[1]:
1. Disperse fresh intestinal secretions in sterile water and coate on a potato agar plate, containing penicillin (100 U/mL)/streptomycin (0.1 mg/mL) to inhibit intestinal bacteria.
2. Culture at 32°C for about 5 days until the development of obvious fungal colonies.
3. Spray DDAO-C6 (100 μM) on colonies and incubate at 32°C for 2 h.
4. Image plate samples on a Amersham Typhoon RGB (λex = 635 nm, λem = 670 ± 15 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.