DMPE-PEG2000-N3
Based on 1 Customer Validation
DMPE-PEG2000-N3 (DMPE-PEG2000-Azide) is a conjugate composed of DMPE, PEG chains, and terminal azido groups (-N3). DMPE-PEG2000-Azide is a click chemistry reagent used for the targeted modification of liposomes/nanoparticles.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 94.87%
-
Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Protocole
- Pureté et documentation
-
Help & FAQs
Activité biologique
Description
In Vitro
DMPE-PEG-Azide is used as a core component in the preparation of liposomes or lipid nanoparticles (LNPs) to encapsulate drugs or genes. Containing the Azide group, DMPE-PEG-Azide can undergo a copper-catalyzed azido-alkynylene cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo a ring-strain-driven alkynylene-azido cycloaddition reaction (SPAAC) with molecules containing DBCO or BCN groups.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
Appearance Solid
-
Color White to off-white
-
SMILES
CCCCCCCCCCCCCC(OC[C@@H](OC(CCCCCCCCCCCCC)=O)COP(OCCNC(OCCOCCN=[N+]=[N-])=O)(O)=O)=O.[n]
-
Synonyms
DMPE-PEG2000-Azide
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 25 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Protocole
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
-
Fiche technique (237 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instruction de manipulation (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DMPE-PEG2000-N3 Related Classifications
HY-182836A Related Classifications
Powered by Bioz