Emimycin
Emimycin has anti-Gram-positive bacteria, negative bacteria and brewer's yeast and other fungal activities.
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- CAS No.: 3735-46-4
- Formule: C4H4N2O2
- Masse moléculaire:112.09
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| MDCK | CC50 |
>100 μM
Compound: 3
|
Cytotoxicity against MDCK cells assessed as reduction in cell viability by MTS assay
Cytotoxicity against MDCK cells assessed as reduction in cell viability by MTS assay
|
[PMID: 29268134] |
Chemical Information
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CAS No. 3735-46-4
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Masse moléculaire 112.09
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Formule C4H4N2O2
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SMILES
O=C1C=[N+]([O-])C=CN1
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Structure Classification
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Initial Source
Streptomyces sp. 2020-I.
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)