LMed 99
LMed 99 is an antiviral agent. LMed 99 inhibits viral RNA synthesis and reduces viral protein expression in a time-dependent manner. LMed 99 potently inhibits Influenza A virus replication. LMed 99 can be used in the research of Influenza A virus infections (H1N1, H5N1).
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Formule: C18H11ClO5
- Masse moléculaire:342.73
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDCK-II | CC50 |
140.93 μM
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Cytotoxicity against Canis familiaris distal renal tube epithelial MDCK II cells assessed via MTT assay with 72 h incubation.
Cytotoxicity against Canis familiaris distal renal tube epithelial MDCK II cells assessed via MTT assay with 72 h incubation.
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42485991 |
| MDCK-II | EC50 |
1.21 μM
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Inhibition of Influenza A virus H1N1 (strain WSN/33/H1N1) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus.
Inhibition of Influenza A virus H1N1 (strain WSN/33/H1N1) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus.
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42485991 |
| MDCK-II | EC50 |
4.91 μM
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Inhibition of Influenza A virus H5N1 (strain A/Thailand/KAN-1/2004) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus.
Inhibition of Influenza A virus H5N1 (strain A/Thailand/KAN-1/2004) replication in Canis familiaris distal renal tube epithelial MDCK II cells assessed via Focus Forming Assay with 24 h co-incubation of reagent and virus.
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42485991 |
In Vitro
The CC50 of LMed 99 (72 h) in MDCK II cells is 140.93 μM, indicating that it exhibits moderate cytotoxicity at high concentrations[1].
LMed 99 (0.4-350 μM; 24 h) potently inhibits the replication of H1N1 in MDCK II cells, with an EC50 of 1.21 μM and a selectivity index as high as 116.4[1].
LMed 99 (9 μM) significantly reduces H1N1 vRNA accumulation at all tested post-infection time points in MDCK II cells, and inhibits viral mRNA synthesis during the early stages of infection (1 and 3 hpi)[1].
LMed 99 (4.5-9 μM; added 4 h post-transfection and incubated for 20 h) enhances the activity of influenza A virus polymerase-related reporter genes in the MDCK II cell minireplicon system, with a 61.37% increase in activity at 4.5 μM and a 108.97% increase at 9 μM[1].
LMed 99 (1.09-17.5 μM) inhibits the replication of highly pathogenic H5N1 in MDCK II cells, with an EC50 of 4.91 μM and a selectivity index of 28.71, showing favorable performance[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDCK II cells
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Concentration:9 μM
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Incubation Time:added at 1 hpi, 3 hpi, or 6 hpi, with incubation until 9 hpi
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Result:Reduced M1 vRNA levels by 82% (n-fold = 0.18) at 1 and 3 hpi, and by 80% (n-fold = 0.20) at 6 hpi.
Reduced M1 mRNA levels by 78% (n-fold = 0.22) at 1 hpi and 70% (n-fold = 0.30) at 3 hpi, with no significant effect at 6 hpi.
Reduced PB1 mRNA levels by 57% (n-fold = 0.43) at 1 hpi and 22% (n-fold = 0.78) at 3 hpi, with no significant effect at 6 hpi.
Chemical Information
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Masse moléculaire 342.73
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Formule C18H11ClO5
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SMILES
ClC1=CC(C=C2C(/C=C/C3=CC=C(C(O)=C3)O)=O)=C(C=C1)OC2=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
LMed 99 Related Classifications
HY-187428 Related Classifications
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