MRC71
MRC71 is a TRIMTAC degrader that recruits endogenous TRIM21 E3 ligase at one end and covalently binds to HaloTag-fusion target proteins at the other end. MRC71 selectively degrades oligomeric CAV1- and Cavin1-mEGFP-Halo, but not monomeric mEGFP-Halo, and exhibits a typical concentration-dependent degradation profile with a hook effect. MRC71 can be used to verify TRIMTAC ternary complex formation and state-selective degradation in cells.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- CAS No.: 3070832-68-4
- Formule: C43H66ClN5O8
- Masse moléculaire:816.47
-
Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Pureté et documentation
- Références
-
Help & FAQs
Activité biologique
Description
In Vitro
MRC71 (2.5-20 μM) drives the formation of a ternary complex between purified TRIM21 PRYSPRY and HaloTag protein in vitro, with a characteristic hook effect occurring at excessively high compound concentrations[1].
MRC71 (0.001-100 μM; 2 h) induces the formation of a dose-dependent ternary complex between full-length TRIM21 and H2B-mEGFP-Halo in RPE-1 cells, with a hook effect occurring at excessively high compound concentrations[1].
MRC71 (0.01-100 μM; 48 h) is a TRIM21-dependent TRIMTAC that selectively degrades oligomerized CAV1-mEGFP-Halo and Cavin1-mEGFP-Halo in RPE-1 cells, but does not affect monomeric mEGFP-Halo[1].
MRC71 (2.5 μM; 24 h) selectively degrades membrane-associated oligomeric Cavin1-mEGFP-Halo via the proteasome in RPE-1 cells, while preserving the cytoplasmic monomer pool and smaller Cavin1-free cleavage products[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 3070832-68-4
-
Masse moléculaire 816.47
-
Formule C43H66ClN5O8
-
SMILES
O=C(C[C@H](N)C1=C(OC)C=CC=C1)N(C2)[C@H](C(N[C@@H](CC3=CC=NC=C3)C(NCCOCCOCCOCCOCCCCCCCl)=O)=O)C[C@H]2C4CCCCC4
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MRC71 Related Classifications
HY-180547 Related Classifications
Powered by Bioz