Cat. No.: HY-N0498

Nitidine chloride

4 Cited Publications

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Based on 4 publication(s) in Google Scholar

Nitidine chloride, a potential anti-malarial lead compound derived from Zanthoxylum nitidum (Roxb) DC, exerts potent anticancer activity through diverse pathways, including inducing apoptosis, inhibiting STAT3 signaling cascade, DNA topoisomerase 1 and 2A, ERK and c-Src/FAK associated signaling pathway, also has anti-inflammatory activity. Nitidine chloride inhibits LPS-induced inflammatory cytokines production via MAPK and NF-kB pathway.

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Pureté: 99.82%
CAS No.: 13063-04-2
Formule: C₂₁H₁₈ClNO₄
Masse moléculaire:383.82

Stockage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Nitidine chloride Chemical Structure

CAS No.: 13063-04-2

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

Plasmodium

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Cellular Effect

Cell Line	Type	Value	Description	References
CEM-SS	EC₅₀	14 μM Compound: Nitidine	Antiviral activity against HIV1 RF in human CEM-SS cells assessed as inhibition of virus-induced cytopathic effect Antiviral activity against HIV1 RF in human CEM-SS cells assessed as inhibition of virus-induced cytopathic effect	[PMID: 11430019]
HCT-116	IC₅₀	4.42 μM Compound: 1c	Antiproliferative activity against human HCT116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 26026362]
HeLa	IC₅₀	0.05 μg/mL Compound: 1	Growth inhibition of human HeLa S3 cells after 72 hrs Growth inhibition of human HeLa S3 cells after 72 hrs	[PMID: 10395504]
HepG2	IC₅₀	3.52 μM Compound: 1c	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 26026362]
HL-60	IC₅₀	3.41 μM Compound: 1c	Antiproliferative activity against human HL60 cells assessed as inhibition of cell viability after 48 hrs by WST-1 assay Antiproliferative activity against human HL60 cells assessed as inhibition of cell viability after 48 hrs by WST-1 assay	[PMID: 26026362]
HT-29	IC₅₀	2.75 μM Compound: 1, 1-k6932	Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay Cytotoxicity against human HT-29 cells after 72 hrs by MTS assay	[PMID: 21435753]
KB	ED₅₀	4.3 μg/mL Compound: Nitidine	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 7130986]
Panel NCI-60 (60 carcinoma cell lines)	GI₅₀	125 nM Compound: Nitidine	Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program	[PMID: 15743190]

In Vitro

Nitidine chloride (0-32 μM, 24-48 h) inhibits the proliferation of A498 and 768-O cells^[1].
Nitidine chloride (8-16 μM, 24 h) induces apoptosis in A498 and 768-O cells^[1].
Nitidine chloride (0-16 μM, 48 h) induces apoptosis in A498 cells, causes cell cycle arrest, and inhibits the ERK pathway^[1].
Nitidine chloride (0-10 μM, 24 h) induces apoptosis in HSC3 and HSC4 cells, inducing apoptosis in OSCC cells through dephosphorylation of STAT3^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

768-O, A498
Concentration:

0, 4, 6, 8, 16, 32 μM
Incubation Time:

24, 48 h
Result:

Inhibited cell proliferation in a time- and dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

768-O, A498
Concentration:

8, 16 μM
Incubation Time:

24 h
Result:

Increased apoptosis rate in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

A498
Concentration:

0, 8, 16 μM
Incubation Time:

48 h
Result:

Increased Bax expression, decreased Bcl-2 expression, increased p53 expression, decreased pro-caspase-3 expression, increased cleaved caspase-3 expression and PARP cleavage level, inhibited ERK1/2, p38 and Akt phosphorylation.

Cell Viability Assay^[2]

Cell Line:

HSC3, HSC4
Concentration:

0, 0.1, 5, 10 μM
Incubation Time:

24 h
Result:

Reduced cell viability.

Western Blot Analysis^[2]

Cell Line:

HSC3, HSC4, OSCC
Concentration:

0, 0.1, 5, 10 μM
Incubation Time:

24 h
Result:

Dephosphorylated STAT3 and induced the cleavage of PARP and caspase-3 in a concentration- and time-dependent manner.

In Vivo

Nitidine chloride (5 mg/kg, intraperitoneal injection, once daily, 5 weeks) inhibits tumor growth in mice and induces apoptosis in renal cancer cells in vivo^[1].
Nitidine chloride (0.375-10 mg/kg, intraperitoneal injection, once daily, 3 weeks) downregulates hypertrophy in mouse myocardium through AGT4B-mediated autophagy^[3].
Nitidine chloride (5-10 mg/kg, intraperitoneal injection, single dose) exerts anti-inflammatory effects in endotoxin-treated mice through Akt-mediated IL-10 production^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

A549 nude mouse xenograft model^[1]
Dosage:

5 mg/kg; daily; 5 weeks
Administration:

Intraperitoneal injection (i.p.)
Result:

Showed non-toxic effects, reduced tumor growth, and induced apoptosis in renal cancer cells in vivo.

Animal Model:

Mice, beagles^[3]
Dosage:

2.5, 5, 10 mg/kg; 0.375, 0.75, 1.5 mg/kg; daily; 4 weeks
Administration:

Intraperitoneal injection (i.p.)
Result:

Both induced dose-dependent cardiac hypertrophy and dysfunction, while medium and high doses led to death in beagle dogs. Cardiac autophagy levels in cardiomyocytes were reduced. Overexpression of ATG4B reversed cardiac hypertrophy and reduced autophagy levels.

Animal Model:

LPS induced endotoxemic mice^[4]
Dosage:

5, 10 mg/kg; single dose
Administration:

Intraperitoneal injection (i.p.)
Result:

Increased IL-10 production, inhibited inflammatory response, reduced mortality, and enhanced Akt activation.

Chemical Information

CAS No. 13063-04-2
Appearance Solid
Masse moléculaire 383.82
Formule C₂₁H₁₈ClNO₄
Color Light yellow to yellow
SMILES

C[N+]1=CC2=CC(OC)=C(OC)C=C2C(C=CC3=C4)=C1C3=CC5=C4OCO5.[Cl-]
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (4)

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Journal Impact Factor

Free Radic Biol Med

Targeting ABCB6 with nitidine chloride inhibits PI3K/AKT signaling pathway to promote ferroptosis in multiple myeloma. [Abstract]

2023 Jul:203:86-101. PMID: 37044150
Life Sci

Nitidine chloride inhibits mTORC1 signaling through ATF4-mediated Sestrin2 induction and targets IGF2R for lysosomal degradation. [Abstract]

2024 Jul 19:122918. PMID: 39034027
PLoS Pathog

G-quadruplex in the TMV Genome Regulates Viral Proliferation and Acts as Antiviral Target of Photodynamic Therapy. [Abstract]

2023 Dec 7;19(12):e1011796. PMID: 38060599
J Pharm Biomed Anal

Integrated network pharmacology and metabolomics to investigate the effect and mechanism of nitidine chloride against cholangiocarcinoma. [Abstract]

2025 Nov 15:265:117063. PMID: 40652810

Solvant et solubilité

In Vitro:

DMSO : 2 mg/mL (5.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* Nitidine chloride is usually formulated as a suspension.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6054 mL

13.0269 mL

26.0539 mL

5 mM

0.5211 mL

2.6054 mL

5.2108 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/0.5% Tween-80 in Saline water

Solubility: 1 mg/mL (2.61 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 15% Cremophor EL 85% Saline

Solubility: 2 mg/mL (5.21 mM); Suspended solution; Need ultrasonic

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

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COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Fang Z, et al. Nitidine chloride induces apoptosis and inhibits tumor cell proliferation via suppressing ERK signaling pathway in renal cancer. Food Chem Toxicol. 2014 Apr;66:210-6. [Content Brief]

[2]. Lee-Han Kim, et al. Nitidine chloride acts as an apoptosis inducer in human oral cancer cells and a nude mouse xenograft model via inhibition of STAT3. Oncotarget. 2017 Aug 24;8(53):91306-91315. [Content Brief]

[3]. Yang Hong, et al. Nitidine chloride induces cardiac hypertrophy in mice by targeting autophagy-related 4B cysteine peptidase. Acta Pharmacol Sin. 2023 Mar;44(3):561-572. [Content Brief]

[4]. Niao Yang, et al. Nitidine chloride exerts anti-inflammatory action by targeting Topoisomerase I and enhancing IL-10 production. Pharmacol Res. 2019 Oct:148:104368. [Content Brief]

[5]. Wang Z, et al. Nitidine chloride inhibits LPS-induced inflammatory cytokines production via MAPK and NF-kappaB pathway in RAW 264.7 cells. J Ethnopharmacol. 2012 Oct 31;144(1):145-50. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6054 mL	13.0269 mL	26.0539 mL	65.1347 mL
DMSO	5 mM	0.5211 mL	2.6054 mL	5.2108 mL	13.0269 mL

Keywords

Nitidine chloride

4 Cited Publications

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Nitidine chloride

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