TAT-MEK1
TAT-MEK1 is an inhibitor ofERK2, consisting of TAT and MEK1 (N-terminal), TAT (YGRKKRRQRRR) derived from human immunodeficiency (HIV-1) transcriptional trans activator (TAT), is a cell-penetrating peptide. TAT-MEK1 IC50 in vitro for ERK2 is 29 μM.
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- CAS No.: 566872-16-0
- Formule: C136H241N53O31S
- Masse moléculaire:3146.77
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
TAT-MEK1 (100 μM, 30 min) can inhibit luciferase activity in NIH.3T3 and PC12 cells , and inhibit the activation of ERK2sup>[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:NIH 3T3 and PC12
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Concentration:100 μM
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Incubation Time:30 min
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Result:Inhibited luciferase activity in cells and the activity of ERK2.
Chemical Information
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CAS No. 566872-16-0
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Masse moléculaire 3146.77
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Formule C136H241N53O31S
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Sequence
Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH2
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Sequence Shortening
GYGRKKRRQRRRGMPKKKPTPIQLNP-NH2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
Références
[1]. Lukanowska M, et al. Bioportides: bioactive cell-penetrating peptides that modulate cellular dynamics. Biotechnol J. 2013 Aug;8(8):918-30. [Content Brief]
[2]. Kelemen BR, et al. Selective in vivo inhibition of mitogen-activated protein kinase activation using cell-permeable peptides. J Biol Chem. 2002 Mar 8;277(10):8741-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TAT-MEK1 Related Classifications
HY-P10074 Related Classifications
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