TSLP-IN-1
Based on 1 Customer Validation
TSLP-IN-1 is a potent thymic stromal lymphopoietin (TSLP) inhibitor. TSLP-IN-1 disrupts TSLP-TSLPR protein-protein interaction, and downregulates IL-4 and IL-13. TSLP-IN-1 can be used for the research of inflammation diseases, such as skin disease, asthma, and allergies.
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- Pureté: 99.67%
- CAS No.: 512837-95-5
- Formule: C18H15NO4S
- Masse moléculaire:341.38
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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IL-4 |
IL-13 |
TSLP-IN-1 (compound C13) (0.25-20 μM; 24 h) selectively inhibits proliferation of Hut78 cells, while showing no cytotoxicity in human keratinocytes and fibroblasts[1].
TSLP-IN-1 (0.25-20 μM; 36 h) inhibits IL-4 and IL-13 release in TSLP-activated HuT78 cells and human primary CD4+T cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary human keratinocytes, dermal fibroblasts, and HuT78 cells
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Concentration:0.25; 0.5; 1; 2.5; 5; 10; 20 μM
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Incubation Time:24 h
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Result:Exhibited strong inhibitory effects at 20 μΜ in Hut78 cells.
Induced no major cytotoxicity in primary human keratinocytes and fibroblasts.
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Cell Line:TSLP-activated HuT78 cells, human
primary CD4+T cells -
Concentration:0.25; 0.5; 1; 2.5; 5; 10; 20 μM
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Incubation Time:36 h
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Result:Significantly reduced TSLP-triggered IL-4 and IL-13 release in a concentration-dependent manner.
Showed strong inhibitory effects at 20 μΜ concentrations in human primary CD4+T cells.
Chemical Information
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CAS No. 512837-95-5
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Appearance Solid
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Masse moléculaire 341.38
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Formule C18H15NO4S
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Color White to off-white
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SMILES
O=C(C1=C(SC2C(N(C3=CC=CC(C)=C3)C(C2)=O)=O)C=CC=C1)O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
DMSO : 50 mg/mL (146.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.32 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9293 mL | 14.6464 mL | 29.2929 mL | 73.2322 mL |
| 5 mM | 0.5859 mL | 2.9293 mL | 5.8586 mL | 14.6464 mL | |
| 10 mM | 0.2929 mL | 1.4646 mL | 2.9293 mL | 7.3232 mL | |
| 15 mM | 0.1953 mL | 0.9764 mL | 1.9529 mL | 4.8821 mL | |
| 20 mM | 0.1465 mL | 0.7323 mL | 1.4646 mL | 3.6616 mL | |
| 25 mM | 0.1172 mL | 0.5859 mL | 1.1717 mL | 2.9293 mL | |
| 30 mM | 0.0976 mL | 0.4882 mL | 0.9764 mL | 2.4411 mL | |
| 40 mM | 0.0732 mL | 0.3662 mL | 0.7323 mL | 1.8308 mL | |
| 50 mM | 0.0586 mL | 0.2929 mL | 0.5859 mL | 1.4646 mL | |
| 60 mM | 0.0488 mL | 0.2441 mL | 0.4882 mL | 1.2205 mL | |
| 80 mM | 0.0366 mL | 0.1831 mL | 0.3662 mL | 0.9154 mL | |
| 100 mM | 0.0293 mL | 0.1465 mL | 0.2929 mL | 0.7323 mL |
TSLP-IN-1 Related Classifications
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