CellTracker CM-DiI  (Synonyms: CM-DiI)

CellTracker CM-DiI (CM-DiI) is a lipophilic fluorochrome that can be used for tracking the migration of lymphocytes. CellTracker CM-DiI is feasible to detect labeled cells in histological sections (excitation 553 nm; emission 570 nm)^[1][2][3].

Guide (The following is our recommended protocol, which is provided as a guide only and should be modified to suit your specific needs).
Using CM-DiI to label lymphocytes to track their migration^[1]
1. Preparation of stock solution
1.1 Dissolve 500 μg of CM-DiI in 250 μL of DMSO.
2. Preparation of working solution
2.1 Transfer 7.5 mL of Hank's Balanced Salt Solution pre-warmed to 37 °C into the DMSO solution containing CM-DiI, and mix for several seconds until the dye is evenly distributed.
3. Specific staining steps
3.1 Collect the overnight lymph fluid and determine the cell concentration. Take an appropriate number of lymphocytes, centrifuge at 4 °C for 10 min, discard the supernatant, and wash the pellet with HBSS.
3.2 Resuspend the washed cells in HBSS.
3.3 Immediately transfer the prepared dye working solution to the cell suspension and mix rapidly using a pipette.
3.4 Incubate the cells at 37 °C for 2 min, followed by an additional 2 min incubation on ice.
3.5 Centrifuge and wash the cells at 4 °C, resuspend them in 20 mL of Lactated Ringer's solution, place the cell suspension on ice, and perform injection within 15-30 min.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1051.49

C₆₈H₁₀₅Cl₂N₃O

180854-97-1

Solid

Orange to red

570

553

CCCCCCCCCCCCCCCCCC[N+]1=C(/C=C/C=C2N(CCCCCCCCCCCCCCCCCC)C3=C(C=CC=C3)C/2(C)C)C(C)(C)C4=C1C=CC(CNC(C5=CC=C(CCl)C=C5)=O)=C4.[Cl-]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Andrade W, et al. The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes. J Immunol Methods. 1996 Aug 14;194(2):181-9.  [Content Brief]

  [2]. Dupuis M, et al. Distribution of adjuvant MF59 and antigen gD2 after intramuscular injection in mice. Vaccine. 1999 Oct 14;18(5-6):434-9.  [Content Brief]

  [3]. Rutten M, et al. Comparison of quantum dots and CM-DiI for labeling porcine autologous bone marrow mononuclear progenitor cells. The Open Stem Cell Journal, 2010, 2(1).