CellTracker Orange CMRA Dye (5/6-mixture)
Based on 1 Customer Validation
CellTracker Orange CMRA Dye is a fluorescent dye. CellTracker Orange CMRA Dye can be used for cell imaging and monitoring the movement and location of cells.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 3031711-06-2
- Formula: C30H25Cl2NO5
- Molecular Weight:550.43
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability assay[1]:
1. Adds 5 μM CellTracker Orange CMRA Dye to each sample at 27-33°C for 30 min to label sample.
2. Centrifuges cells 1000 g for 10 min, resuspends cell pellet in pre-warmed 10% iFCS RPMI (3*107cells/Ml).
3. Incubates cells at 27-33°C for 30 min.
4. Centrifuges cells 1000 g for 10 min and wash with pre-warmed PBS without Ca2+ and Mg2+.
5. Centrifuges cells 1000 g for 10 min and cells resuspends in pre-warmed 10% iFCS RPMI.
6. Measure fluorescence intensity (Ex=548 nm, Em=576 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
576
548
Chemical Information
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CAS No. 3031711-06-2
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Appearance Solid
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Molecular Weight 550.43
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Formula C30H25Cl2NO5
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Color Off-white to pink
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SMILES
O=C(C)OC1=C(Cl)C=C(C2(C(C=CC(CCl)=C3)=C3C(O2)=O)C(C=C(C(C)=CC(C)(C)N4C)C4=C5)=C5O6)C6=C1.O=C(C)OC7=C(Cl)C=C(C8(C(C=C(CCl)C=C9)=C9C(O8)=O)C(C=C(C(C)=CC(C)(C)N%10C)C%10=C%11)=C%11O%12)C%12=C7
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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