360A
Based on 4 publication(s) in Google Scholar
360A is a selective stabilizer of G-quadruplex, and also inhibits telomerase activity with an IC50 of 300 nM for telomerase in TRAP-G4 assay.
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- CAS. Nr.: 794458-56-3
- Formel: C27H23N5O2
- Molecular Weight:449.50
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Publications Citing Use of MedChemExpress (MCE) 360A
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Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | IC50 |
45 nM
Compound: 360A
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Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay
Inhibition of human telomerase expressed in in HEK293T cells in presence of primer (T2AG3)4 by primer extension assay
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[PMID: 17954919] |
| HEK-293T | IC50 |
52 nM
Compound: 360A
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Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay
Inhibition of human telomerase expressed in HEK293T cells treated before telomerase elongation by telomeric repeat amplification protocol assay
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[PMID: 17954919] |
| HEK-293T | IC50 |
5600 nM
Compound: 360A
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Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay
Inhibition of human telomerase expressed in HEK293T cells in presence of primer (T2AG3)3 by primer extension assay
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[PMID: 17954919] |
| HEK-293T | IC50 |
63 nM
Compound: 360A
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Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay
Inhibition of human telomerase expressed in HEK293T cells treated after telomerase elongation by telomeric repeat amplification protocol assay
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[PMID: 17954919] |
| HEK-293T | IC50 |
8400 nM
Compound: 360A
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Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay
Inhibition of human telomerase expressed in HEK293T cells in presence of primer TS by primer extension assay
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[PMID: 17954919] |
| HeLa | IC50 |
8.5 μM
Compound: 360A
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Anti-tumor activity against human HeLa cells incubated for 72 hrs by MTT assay
Anti-tumor activity against human HeLa cells incubated for 72 hrs by MTT assay
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[PMID: 38215623] |
| HFF-1 | IC50 |
30.6 μM
Compound: 360A
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Cytotoxicity against against human HFF-1 cells incubated for 72 hrs by MTT assay
Cytotoxicity against against human HFF-1 cells incubated for 72 hrs by MTT assay
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[PMID: 38215623] |
| HT-29 | IC50 |
>100 μM
Compound: 360A
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Anti-tumor activity against human HT-29 cells incubated for 72 hrs by MTT assay
Anti-tumor activity against human HT-29 cells incubated for 72 hrs by MTT assay
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[PMID: 38215623] |
| PC-3 | IC50 |
2.5 μM
Compound: 360A
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Anti-tumor activity against human PC-3 cells incubated for 72 hrs by MTT assay
Anti-tumor activity against human PC-3 cells incubated for 72 hrs by MTT assay
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[PMID: 38215623] |
360A inhibits telomerase activity and stabilizes G-quadruplex, with an IC50 of 300 nM for telomerase in TRAP-G4 assay. 360A reduces the viability of glioma cell lines, such as T98G, CB193, U118-MG, SAOS-2 and Primary astrocytes, with IC50s of 4.8 ± 1.1 μM, 3.9 ± 0.4 μM, 8.4 ± 0.5 μM, >15 μM and 17.4 ± 1.2 μM, respectively[1]. 360A causes Rad51-dependent telomere aberrations preferentially involving the lagging strand telomeres, including telomere losses or telomere doublets, and induces DNA-PKcs-dependent sister telomere fusions[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 794458-56-3
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Molecular Weight 449.50
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Formel C27H23N5O2
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SMILES
O=C(C1=CC=CC(C(NC2=C[N+](C)=C3C=CC=CC3=C2)=O)=N1)NC4=C[N+](C)=C5C=CC=CC5=C4
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (4)
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Journal Impact Factor
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Most Recent
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Nucleic Acids Res
Guanine quadruplexes in the RNA genome of the tick-borne encephalitis virus: their role as a new antiviral target and in virus biology. [Abstract]2022 May 6;50(8):4574-4600. PMID: 35420134 -
Cell Death Dis
2021 Oct 25;12(11):999. PMID: 34697294 -
Int J Biol Macromol
2025 Mar:296:139582. PMID: 39798757 -
Protokoll
The cell proliferation reagent WST-1 assay is performed. In brief, cells are seeded at various densities, depending on cell type (0.25-4 × 103 cells/well in 100 μL complete medium), in 96-well culture plates and treated with various concentrations (0.1-20 μM) of 360A or the corresponding concentrations of DMSO (control wells) for 3 or 7 days at 37°C in an atmosphere containing 5% CO2. For 7-day assays, the medium is changed on day 3. Experiments are performed in triplicate[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
Verweise
[1]. Pennarun G, et al. Apoptosis related to telomere instability and cell cycle alterations in human glioma cells treated by new highly selective G-quadruplex ligands. Oncogene. 2005 Apr 21;24(18):2917-28. [Content Brief]
[2]. Gauthier LR, et al. Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death. Cell Mol Life Sci. 2012 Feb;69(4):629-40. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
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