AN0128
Based on 1 Customer Validation
AN0128 is a boron-containing antibacterial and anti-inflammatory agent. AN0128 against S. aureus, S. epidermidis, P. acnes, B. subtilis with minimum inhibitory concentration (MIC) values of 1, 0.5, 0.3, 1 μg/mL. AN0128 can be used for the research of periodontal disease and cutaneous diseases.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.95%
- CAS. Nr.: 872044-70-7
- Formel: C20H16BCl2NO3
- Molecular Weight:400.06
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
AN0128 (compound 2g; 10 μM; 24-48 hours) shows strong inhibition of the release of pro-inflammatory cytokines (TNF-α, IL-1β) but no inhibition of IFN-γ or IL-4 release in human peripheral blood mononuclear cells (PBMCs)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Experimental periodontitis in twelve-week-old male Sprague-Dawley rats (weighing from 275 to 300 g each)[2]
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Dosage:1% AN0128 in 40% Transcutol P, 40% PBS, and 20% ethanol vehicle
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Administration:Daily topical daub for 7 days
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Result:Similar to Ketorolac, resulting 50% and 35% increase in bone area and bone volume respectively, and 38%, 42% decrease in bone loss and inflammation respectively.
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Animal Model:Experimental periodontitis in twelve-week-old male Sprague-Dawley rats (weighing from 275 to 300 g each)[2]
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Dosage:5% with Total toothpaste
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Administration:Applied daily via a cotton-tip applicator. for 7 days
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Result:Had more effect than Total toothpaste, resulting in a 33% increase in bone volume, and a 47% decrease in bone loss.
Chemical Information
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CAS. Nr. 872044-70-7
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Appearance Solid
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Molecular Weight 400.06
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Formel C20H16BCl2NO3
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Color White to off-white
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SMILES
OC1=CC=CN=C1C(OB(C2=CC(Cl)=C(C)C=C2)C3=CC(Cl)=C(C)C=C3)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 125 mg/mL (312.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (290 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Baker SJ, et al. Identification of a novel boron-containing antibacterial agent (AN0128) with anti-inflammatory activity, for the potential treatment of cutaneous diseases. Bioorg Med Chem Lett. 2006 Dec 1;16(23):5963-7. [Content Brief]
[2]. Luan Q, et al. Inhibition of experimental periodontitis by a topical boron-based antimicrobial. J Dent Res. 2008 Feb;87(2):148-52. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4996 mL | 12.4981 mL | 24.9963 mL | 62.4906 mL |
| 5 mM | 0.4999 mL | 2.4996 mL | 4.9993 mL | 12.4981 mL | |
| 10 mM | 0.2500 mL | 1.2498 mL | 2.4996 mL | 6.2491 mL | |
| 15 mM | 0.1666 mL | 0.8332 mL | 1.6664 mL | 4.1660 mL | |
| 20 mM | 0.1250 mL | 0.6249 mL | 1.2498 mL | 3.1245 mL | |
| 25 mM | 0.1000 mL | 0.4999 mL | 0.9999 mL | 2.4996 mL | |
| 30 mM | 0.0833 mL | 0.4166 mL | 0.8332 mL | 2.0830 mL | |
| 40 mM | 0.0625 mL | 0.3125 mL | 0.6249 mL | 1.5623 mL | |
| 50 mM | 0.0500 mL | 0.2500 mL | 0.4999 mL | 1.2498 mL | |
| 60 mM | 0.0417 mL | 0.2083 mL | 0.4166 mL | 1.0415 mL | |
| 80 mM | 0.0312 mL | 0.1562 mL | 0.3125 mL | 0.7811 mL | |
| 100 mM | 0.0250 mL | 0.1250 mL | 0.2500 mL | 0.6249 mL |
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