BMPO
Based on 3 publication(s) in Google Scholar
BMPO (BocMPO) is a cell-permeable superior spin trap with favorable chemical and spectroscopic features. BMPO (BocMPO) can be used for detecting thiyl radicals, hydroxyl radicals, superoxide anions and glutathiyl radicals.
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- Reinheit: 98.95%
- CAS. Nr.: 387334-31-8
- Formel: C10H17NO3
- Molecular Weight:199.25
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Speicherung:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BMPO
More
Biologische Aktivität
BMPO is able to trap both OH and GS to form more persistent BMPO/ OH and BMPO/ SG adducts. In addition, the electron spin resonance (ESR) spectra of BMPO-derived adducts exhibit high signal-to-noise ratios in biological systems. For example, the ESR spectrum of the BMPO glutathionyl adduct (BMPO/ SG) does not fully overlap with the spectrum of its hydroxyl adduct. The favorable chemical and spectroscopic features make BMPO ideal for the detection of superoxide anions, hydroxyl and thiyl radicals.[1]The potential toxic effects of the spin trap BMPO is measured by two estimates of cell viability (trypan blue exclusion and colony formation).[3]BMPO has no significant effect on cell viability at 2.5 or 25 mM in CHO cells. However, it leads to a significant increase in the number of cells that are unable to exclude Trypan blue at 50 mM BMPO. BMPO significantly reduces a 30% reduction in colony formation at 25 mM concentration in CHO cells. And, BMPO completely inhibits the colony formation of 9L tumor cells at 50 mM.[3]The cell function (rate of oxygen consumption) is also measured, BMPO (25 mM) significantly reduces the oxygen consumption rates in CHO cells.[3]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 387334-31-8
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Appearance Solid
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Molecular Weight 199.25
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Formel C10H17NO3
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Color Off-white to light yellow
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SMILES
O=C(C1(C)[N+]([O-])=CCC1)OC(C)(C)C
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Synonyms
BocMPO
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Dual-functional cationic hydrogel engineered for simultaneous prevention of postoperative tumor recurrence and wound infection. [Abstract]2026 Apr 29;24(1):575. PMID: 42057107 -
J Hazard Mater
Enhanced antibiotic degradation in micro-nanobubble-assisted dielectric barrier discharge systems: The role of gas-liquid interfaces in ROS exposure. [Abstract]2026 Jun 1:510:142167. PMID: 42025070 -
ACS Appl Mater Interfaces
Dinuclear Ir(III)-Oligomer as a Sunlight-Driven Hydroxyl Radical Generator for Effective Cancer Photodynamic Therapy. [Abstract]2025 Jun 11;17(23):33465-33473. PMID: 40456027
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (501.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (12.55 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. H Zhao, et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic Biol Med [Content Brief]
[2]. Hongtao Zhao, et al. Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci U S A [Content Brief]
[3]. Nadeem Khan, et al. Spin traps: in vitro toxicity and stability of radical adducts. Free Radic Biol Med. 2003 Jun 1;34(11):1473-81. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.0188 mL | 25.0941 mL | 50.1882 mL | 125.4705 mL |
| 5 mM | 1.0038 mL | 5.0188 mL | 10.0376 mL | 25.0941 mL | |
| 10 mM | 0.5019 mL | 2.5094 mL | 5.0188 mL | 12.5471 mL | |
| 15 mM | 0.3346 mL | 1.6729 mL | 3.3459 mL | 8.3647 mL | |
| 20 mM | 0.2509 mL | 1.2547 mL | 2.5094 mL | 6.2735 mL | |
| 25 mM | 0.2008 mL | 1.0038 mL | 2.0075 mL | 5.0188 mL | |
| 30 mM | 0.1673 mL | 0.8365 mL | 1.6729 mL | 4.1824 mL | |
| 40 mM | 0.1255 mL | 0.6274 mL | 1.2547 mL | 3.1368 mL | |
| 50 mM | 0.1004 mL | 0.5019 mL | 1.0038 mL | 2.5094 mL | |
| 60 mM | 0.0836 mL | 0.4182 mL | 0.8365 mL | 2.0912 mL | |
| 80 mM | 0.0627 mL | 0.3137 mL | 0.6274 mL | 1.5684 mL | |
| 100 mM | 0.0502 mL | 0.2509 mL | 0.5019 mL | 1.2547 mL |
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