Art. -Nr.: HY-117280

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GN25

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GN25 is a specific p53-Snail binding inhibitor with antitumor effects.

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Reinheit: 98.55%
CAS. Nr.: 1227401-27-5
Formel: C₁₅H₁₄O₆S
Molecular Weight:322.33

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

GN25 Chemical Structure

CAS. Nr.: 1227401-27-5

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

GN25 is a specific p53-Snail binding inhibitor with antitumor effects^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	28.7 μM Compound: 14; GN25	Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay Cytotoxicity against human HL60 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 31563013]
Jurkat	IC₅₀	21.3 μM Compound: 14; GN25	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay Cytotoxicity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 31563013]
MONO-MAC-6	IC₅₀	12.8 μM Compound: 14; GN25	Cytotoxicity against human MONO-MAC-6 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay Cytotoxicity against human MONO-MAC-6 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 31563013]
THP-1	IC₅₀	33.3 μM Compound: 14; GN25	Cytotoxicity against human THP1 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay Cytotoxicity against human THP1 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 31563013]
U-937	IC₅₀	26.6 μM Compound: 14; GN25	Cytotoxicity against human U937 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay Cytotoxicity against human U937 cells assessed as reduction in cell viability incubated for 24 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 31563013]

In Vitro

GN25 (10 and 20 μM; 24 h) inhibits cell viability of K-Ras-mutated MEF cells^[1].
GN25 (5 μM; 4 h) activates p53 in a K-Ras-dependent manner^[1].
GN25 (1-10 μM; 1-6 h) induces p53 and p21 in a dose- and time- dependent manner in K-Ras-mutated A549 and HCT116 cell lines^[1].
GN25 activates wild-type p53 in p53^WT/MT cells^[1].
GN25 (0.25 μM) recovers the masking effect of p53 on CK1/GSK3b-mediated Snail phosphorylation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HCT116, A459 and MKN45 cell lines
Concentration:

10 μM
Incubation Time:

24 h
Result:

Obviously suppressed the cell viability of HCT116 and A459 cell lines.

Cell Proliferation Assay^[1]

Cell Line:

K-Ras-transformed and N-Ras/Myc-transformed MEF cells
Concentration:

10 μM and 20 μM
Incubation Time:

24 h
Result:

Inhibited the viability of K-Ras-transformed but not N-Ras/Myc-transformed MEF cells.

Western Blot Analysis^[1]

Cell Line:

K-Ras-MEF, N-Ras/Myc-MEF and Nontransformed MEF cells
Concentration:

5 μM
Incubation Time:

4 h
Result:

Only induced p53 and p21 in K-Ras-MEF cells.

Western Blot Analysis^[1]

Cell Line:

K-Ras-mutated A549, HCT116 cell lines and MKN45
Concentration:

1, 5, and 10 μM
Incubation Time:

1, 3, and 6 h
Result:

Induced p53 and p21 in a dose- and time- dependent manner in A549 and HCT116 cells.

In Vivo

GN25 (10 and 20 mg/kg; i.p. once a week for 10 weeks) blocks the tumor progression and induces tumor regression in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Athymic mice with A549 xenografts^[1]
Dosage:

10 and 20 mg/kg
Administration:

Intraperitoneal injection; once a week for 10 weeks
Result:

Obviously regressed tumors in mice. Showed no significant toxicity in liver, pancreas and kidney.

Chemical Information

CAS. Nr. 1227401-27-5
Appearance Solid
Molecular Weight 322.33
Formel C₁₅H₁₄O₆S
Color Light yellow to orange
SMILES

O=C(O)CCSC(C1=O)=CC(C2=C1C(OC)=CC=C2OC)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (310.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1024 mL

15.5121 mL

31.0241 mL

5 mM

0.6205 mL

3.1024 mL

6.2048 mL

10 mM

0.3102 mL

1.5512 mL

3.1024 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lee SH, Shen GN, Jung YS, Lee SJ, Chung JY, Kim HS, Xu Y, Choi Y, Lee JW, Ha NC, Song GY, Park BJ. Antitumor effect of novel small chemical inhibitors of Snail-p53 binding in K-Ras-mutated cancer cells. Oncogene. 2010 Aug 12;29(32):4576-87. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.1024 mL	15.5121 mL	31.0241 mL	77.5603 mL
	5 mM	0.6205 mL	3.1024 mL	6.2048 mL	15.5121 mL
	10 mM	0.3102 mL	1.5512 mL	3.1024 mL	7.7560 mL
	15 mM	0.2068 mL	1.0341 mL	2.0683 mL	5.1707 mL
	20 mM	0.1551 mL	0.7756 mL	1.5512 mL	3.8780 mL
	25 mM	0.1241 mL	0.6205 mL	1.2410 mL	3.1024 mL
	30 mM	0.1034 mL	0.5171 mL	1.0341 mL	2.5853 mL
	40 mM	0.0776 mL	0.3878 mL	0.7756 mL	1.9390 mL
	50 mM	0.0620 mL	0.3102 mL	0.6205 mL	1.5512 mL
	60 mM	0.0517 mL	0.2585 mL	0.5171 mL	1.2927 mL
	80 mM	0.0388 mL	0.1939 mL	0.3878 mL	0.9695 mL
	100 mM	0.0310 mL	0.1551 mL	0.3102 mL	0.7756 mL

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GN25

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Anti-Pancreatic Cancer Compound Library

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Transcription Factor-Targeted Library

Highly Selective Activators Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

GN25

Apoptosis Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Ferroptosis Compound Library

Pyroptosis Compound Library

Glutamine Metabolism Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Transcription Factor-Targeted Library

Highly Selective Activators Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

GN25 Related Classifications

HY-117280 Related Classifications