VHL-SNAP2-5C
Based on 1 Customer Validation
VHL-SNAP2-5C is a PROTAC degrader targeting SNAP-tag fusion proteins, with an IC50 of 0.33 μM for binding to VHL. VHL-SNAP2-5C covalently binds to the SNAP-tag target at one end and recruits the VHL ubiquitin E3 ligase at the other end to form a ternary complex, thereby inducing proteasome-dependent ubiquitination and degradation of SNAP fusion proteins through its heterobifunctional structure. VHL-SNAP2-5C can be used in studies of protein homeostasis and degradation mechanisms.
(Pink: SNAP fusion protein ligand (HY-W128709); Blue: VHL ligand (HY-112078); Black: linker (HY-Y1139)).
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.98%
- Formel: C42H53ClN8O6S
- Molecular Weight:833.44
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| THP-1 | IC50 |
>50 μM
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Cytotoxicity against human monocytic THP1 cells measured via Resazurin reagent.
Cytotoxicity against human monocytic THP1 cells measured via Resazurin reagent.
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39444693 |
In Vitro
VHL-SNAP2-5C (0.1-100 μM; 24 h) induces target protein degradation in HEK293 SNAP-EGFP cells[1].
VHL-SNAP2-5C (1 μM; 2-8 h) binds to free target proteins and induces their degradation in HEK293 SNAP-EGFP cells[1].
VHL-SNAP2-5C (0.1-5.0 μM; 2-24 h) induces degradation in HAP1 SNAP-CLCaEN cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HAP1 SNAP-CLCaEN
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Concentration:0.1, 0.2, 1.0, 2.0, 5.0 μM
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Incubation Time:2 h, 24 h
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Result:Depleted the target protein nearly below the detection limit at 24 h.
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Cell Line:HAP1 SNAP-CLCaEN
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Concentration:1 μM
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Incubation Time:24 h
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Result:Did not cause bulk structural differences or disassembly in the actin cytoskeleton.
Chemical Information
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Appearance Solid
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Molecular Weight 833.44
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Formel C42H53ClN8O6S
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Color White to off-white
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SMILES
O=C(NCC1=CC=C(COC2=NC(N)=NC(Cl)=C2)C=C1)CCCCCC(N[C@@H](C(C)(C)C)C(N3[C@H](C(N[C@H](C4=CC=C(C5=C(C)N=CS5)C=C4)C)=O)C[C@@H](O)C3)=O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (119.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Reinheit & Dokumentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1998 mL | 5.9992 mL | 11.9985 mL | 29.9962 mL |
| 5 mM | 0.2400 mL | 1.1998 mL | 2.3997 mL | 5.9992 mL | |
| 10 mM | 0.1200 mL | 0.5999 mL | 1.1998 mL | 2.9996 mL | |
| 15 mM | 0.0800 mL | 0.3999 mL | 0.7999 mL | 1.9997 mL | |
| 20 mM | 0.0600 mL | 0.3000 mL | 0.5999 mL | 1.4998 mL | |
| 25 mM | 0.0480 mL | 0.2400 mL | 0.4799 mL | 1.1998 mL | |
| 30 mM | 0.0400 mL | 0.2000 mL | 0.3999 mL | 0.9999 mL | |
| 40 mM | 0.0300 mL | 0.1500 mL | 0.3000 mL | 0.7499 mL | |
| 50 mM | 0.0240 mL | 0.1200 mL | 0.2400 mL | 0.5999 mL | |
| 60 mM | 0.0200 mL | 0.1000 mL | 0.2000 mL | 0.4999 mL | |
| 80 mM | 0.0150 mL | 0.0750 mL | 0.1500 mL | 0.3750 mL | |
| 100 mM | 0.0120 mL | 0.0600 mL | 0.1200 mL | 0.3000 mL |
VHL-SNAP2-5C Related Classifications
HY-175417 Related Classifications
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