country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-135368

Copy Link Print Information

8pyDTZ

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase. 8pyDTZ exhibits spectrally shifted emission. 8pyDTZ has excellent biocompatibility and superior in vivo sensitivity. 8pyDTZ can be used for in vivo luminescence imaging.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 2351898-91-2
Formula: C₂₄H₁₈N₄O
Molecular Weight:378.43

Storage:

Powder -20°C, 3 years , 4°C, 2 years

* The compound is unstable in solutions, freshly prepared is recommended.

8pyDTZ Chemical Structure

CAS No.: 2351898-91-2

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Imaging Procedure^[2]
1. Anesthetize the mouse and remove hair from the imaging area, then disinfect with 70% ethanol.
2. Inject the mouse intraperitoneally with 8pyDTZ working solution.
3. Immediately place the mouse in a bioluminescence imaging device and set the parameters.
4. Acquire bioluminescence images for 20 minutes.
5. Analyze the images using software such as ImageJ.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

LumiLuc-8pyDTZ pair is compared with several benchmark reporters in a tumor xenograft mouse model. LumiLuc-8pyDTZ shows high sensitivity and does not need organic cosolvents for in vivo administration. LumiScarlet-8pyDTZ is comparable to Akaluc-AkaLumine, the brightest ATP-dependent luciferase-luciferin pair, for detecting cells in deep tissues of mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2351898-91-2
Appearance Solid
Molecular Weight 378.43
Formula C₂₄H₁₈N₄O
Color Light brown to brown
SMILES

O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=NC=C4)NC(C5=CC=CC=C5)=CN31
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (3)

All

Sort by:

Journal Impact Factor

Nature

De novo design of luciferases using deep learning. [Abstract]

2023 Feb;614(7949):774-780. PMID: 36813896
Proc Natl Acad Sci U S A

Targeted glycan degradation potentiates cellular immunotherapy for solid tumors. [Abstract]

2023 Sep 19;120(38):e2300366120. PMID: 37695897
University of California

2023 Nov.

Solvent & Solubility

In Vitro:

EtOH + HCl : 2 mg/mL (5.28 mM; ultrasonic and adjust pH to 2 with 1M HCl; DMSO can inactivate 8pyDTZ's activity)

H₂O : < 0.1 mg/mL (insoluble; DMSO can inactivate 8pyDTZ's activity)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6425 mL

13.2125 mL

26.4250 mL

5 mM

0.5285 mL

2.6425 mL

5.2850 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yeh HW, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
EtOH + HCl	1 mM	2.6425 mL	13.2125 mL	26.4250 mL	66.0624 mL
EtOH + HCl	5 mM	0.5285 mL	2.6425 mL	5.2850 mL	13.2125 mL

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote