Fl-DIBO
Based on 1 Customer Validation
Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe to azide compounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference. Fl-DIBO is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity: 99.60%
- CAS No.: 1407523-31-2
- Formula: C19H12O3
- Molecular Weight:288.30
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended experimental protocol. This protocol is provided as a reference only and should be adjusted according to your specific needs).
Labeling of diazo-tagged proteins[2][3]:
1. Resuspend the proteins in 1×PBS (pH 7.4), and incubate with NHS-activated ester (25 mM, dissolved in DMSO) overnight at room temperature.
2. Co-incubate the labeled proteins with Fl-DIBO (25-500 μM) at 37 °C for 18 h.
3. Analyze the proteins by SDS-PAGE gel electrophoresis and in-gel fluorescence imaging analysis, λex=365 nm, λem=480 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1407523-31-2
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Appearance Solid
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Molecular Weight 288.30
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Formula C19H12O3
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Color Light yellow to yellow
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SMILES
O=C1C2=C1C3=CC=C(OC)C=C3C#CC4=C2C=CC(OC)=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
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Data Sheet (267 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ke Wang, et al. A metal-free turn-on fluorescent probe for the fast and sensitive detection of inorganic azides. Bioorg Med Chem Lett. 2016 Apr 1;26(7):1651-4. [Content Brief]
[2]. Frédéric Friscourt, et al. Fluorogenic Strain-Promoted Alkyne-Diazo Cycloadditions. Chemistry. 2015 Sep 28;21(40):13996-4001. doi: 10.1002/chem.201502242. Epub 2015 Aug 18. [Content Brief]
[3]. Camille Favre, et al. Sydnone Reporters for Highly Fluorogenic Copper-Free Click Ligations. J Org Chem. 2018 Feb 16;83(4):2058-2066. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fl-DIBO Related Classifications
HY-D1506 Related Classifications
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