Oxymethurea

Oxymethurea (Dimethylolurea) is a positive mutagen in the in vitro Ames test, which correctly predicts its mutagenicity, whereas TOPKAT and CASETOX incorrectly predict it as a non-mutagen^[1].
Oxymethurea (DMU; DMU/BSA mass ratio of 2:5; pH 9.0; room temperature) modifies tyrosine residues in BSA in a time-dependent manner, with modification rates of approximately 14%, 31% and 70% at 4, 8 and 24 h, respectively^[2].
Oxymethurea (0.21-8.33 μmol per plate; 30 min pre-incubation, 48 h or 72 h incubation) induces concentration-dependent mutagenicity in Salmonella typhimurium strains TA98, TA100 and TA102 with or without metabolic activation; at concentrations of 2.08 to 8.33 μmol per plate, the number of revertant colonies doubles^[3].
Oxymethurea (3.3-333 μmol/l; 48 h without S9, 3 h with S9 plus 45 h additional culture) induces a weak concentration-dependent increase in micronuclei in V79 Chinese hamster cells only under non-metabolically activated conditions, with the micronucleus rate reaching 3.0% at the concentration of 333 μmol/l, but shows no effect in the presence of metabolic activation^[3].
Oxymethurea (up to 10 mmol/l; 15 min) does not affect the assembly of tubulin isolated from porcine brain into microtubules^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.