Ac4GlcNAcF3
Ac4GlcNAcF3 is a cell-permeable glycomimetic that can be recognized and utilized by OGT and is resistant to hydrolysis by OGA. GlcNAcF3, the active metabolite of Ac4GlcNAcF3, can be recognized by OGT as a substrate for peptide modification. Ac4GlcNAcF3 upregulates the level of cellular O-GlcNAc modification. Ac4GlcNAcF3 can be used in studies related to glycosylation regulation.
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- CAS No.: 137766-83-7
- Formule: C16H20F3NO10
- Masse moléculaire:443.33
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Ac4GlcNAcF3 produces GlcNAcF3 as its active metabolite, which is recognized by OGT as a substrate for peptide modification in peptide-based in vitro assays but cannot be cleaved by OGA[1].
Ac4GlcNAcF3 (0-100 μM; 0-36 h) significantly increases the level of O-GlcNAcylation in NIH3T3 cells[1].
Ac4GlcNAcF3 (20 μM; 24 h) exerts an OGT expression-dependent O-GlcNAcylation-enhancing effect in NIH3T3 cells, as reduced OGT levels significantly attenuate this effect[1].
Ac4GlcNAcF3 (0-100 μM; 48 h) exhibits no significant cytotoxicity against NIH3T3, MCF7, HepG2 or HeLa cells[1].
Ac4GlcNAcF3 (20 μM; 24 h) increases the level of O-GlcNAc glycosylation in NIH3T3 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:NIH3T3 cells
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Concentration:0, 5, 10, 20, 50, 100 μM
20 μM -
Incubation Time:24 h
12, 24, 36 h -
Result:Significantly increased cellular O-GlcNAcylation levels in a concentration-dependent manner.
Achieved the highest O-GlcNAcylation level at 20 μM after 24 h incubation.
Produced the maximal O-GlcNAcylation-enhancing effect at 20 μM for 24 h over tested time points.
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Cell Line:siRNA-transfected NIH3T3 cells
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Concentration:20 μM
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Incubation Time:24 h
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Result:Showed a significant decrease in O-GlcNAcylation levels in siOGT-transfected cells compared to siCON-transfected cells.
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Cell Line:NIH3T3, MCF7, HepG2, and HeLa cells
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Concentration:0, 5, 10, 20, 50, 100 μM
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Incubation Time:48 h
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Result:Showed no significant cytotoxicity in any tested cell lines at concentrations up to 50 μM.
Maintained viability near control levels across the 0-50 μM range.
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Cell Line:NIH3T3 cells
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Concentration:20 μM
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Incubation Time:24 h
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Result:Increased cellular O-GlcNAcylation levels.
Exhibited a slightly weaker O-GlcNAcylation-enhancing effect than OGA inhibitors PUGNAc (HY-108241) and Thiamet-G (HY-12588) at the same concentration and incubation time.
Chemical Information
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CAS No. 137766-83-7
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Masse moléculaire 443.33
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Formule C16H20F3NO10
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SMILES
O(C(C)=O)[C@@H]1[C@@H](NC(C(F)(F)F)=O)C(OC(C)=O)O[C@H](COC(C)=O)[C@H]1OC(C)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ac4GlcNAcF3 Related Classifications
HY-W699962 Related Classifications
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